In this study , map2 and sap2 , two homologs ofap2 in arabidopsis and hap2 in hyacinthus orientalis l . , were isolated from the flower buds of apple ( mains pumila mill . ) 拟南芥的ap2和ant基因、玉米的gl15 、 ids1基因、矮牵牛的phap2a 、 phap2b基因以及风信子的hap2基因都属于这一基因家族。
A cdna labrary was constructed by using the floral meristem materials from the explants cultured on the medium containing hormones for 5 days and 10 days . screenings of cdna library was carried out by a microarray technique . two probes were used 本研究利用风信子离体实验系统，从分离生长素和细胞分裂素诱导响应的基因着手，为初步理解两种激素在花发育过程中的作用机理提供新资料。
Floral organs have been successfully induced from the regenerated floral buds of ffyacinthus orienralis l . by precisely controlling exogenous hormones in the medium . under high concentration of cytokinin and auxin , the regenerated floral bud produces only tepals 以风信子花被片为外植体，通过控制培养基中细胞分裂素和生长素的浓度，可诱导花芽的再生和控制产生花器官的种类，建立起离体花器官定向控制系统。
A homolog of ga sbeta - hydroxylase gene expression pattern was examined . hybridization signals of this gene could be detected at day 5 and however , they appear again after day 10 when explants cultured with hormones . it has the similar pattern when the explants cultured without hormones or uncultured 风信子ga羟基化酶类似基回在外植体表达，在含有外源撒素条件下培养的前5天受到激素的抑制而几乎不表达，第10天开始在花分生组织中表达。
On the toilet table the bouquets - roses , lilacs and hyacinths - appeared like a very ruin of flowers . their perfume was strong and penetrating , while through the dampish air of the place , which was full of the spoiled exhalations of the washstand , came occasional whiffs of a more pungent scent , the scent of some grains or dry patchouli ground to fine powder at the bottom of a cup 梳妆台上放着许多花束，有玫瑰，丁香，风信子，花堆得像要坍塌下来，散发着一股浓郁的沁人心脾的芳香室内空气潮湿，洗脸池中散发出的一股淡淡气味中，不时飘出一阵刺鼻的香味，那是从一只高脚杯底部的九根捏碎了的干广藿香茎中发出来的。
By combining the information from the cdna sequences with functions of their homologs , we suggest that two kinds of hormones may play important roles during flower regeneration in a few ways : ( i ) proteolytic system of ubiqutin / 26s proteosome complex , ( ii ) actin - dependent vesicular cycling of auxin efflux carrier , ( iii ) regulating transcription of genes , ( iv ) phosphorylation and dephosphorylation 经过进一步的筛选，结合序列特征和同源基因的功能信息对这些基因功能进行了初步分析。认为两种外源激素可能通过泛素26s蛋白酶体复合体对调节蛋白的降解机制，生长素运输载体肌动蛋白依赖的小泡运输，基因转录调节，磷酸化和去磷酸化等调节过程诱导风信子花分生组织的再生。
Positive probe was made from the floral meristem materials cultured for 5 days and 10 days with hormones by rna reverse transcription and labelling with radioactive dctp ; two negative probes from uncultured explants and it cultured for 5 days and 10 days . then , we did calculating signal arrays , sequencing , sequence analysis and alignments on genebank etc . finally 229 different ests were got from the cdna library 用在含有外源激素的培养基上培养5天(花分生组织开始形成)和10天(花分生组织已基本形成)的风信子外植体为材料，构建起cdna文库。利用cdnamicroarrray技术，经过杂交筛选和est分析序列分析，最终从cdna文库中获得了229个不同的外源激素诱导响应的基因序列。
