11 pgcs were isolated from 55d goat and cloned to seconed passage , nor pgcs from 70th day goat embryonic genital . the presence of lif had no positive influence on the isolation of es - like cell 55d的胎儿获得11个pgcs集落，可传代2次，从70d胚胎的生殖腺没有分离到pgcs细胞集落。
Two pgcs were cloned to forth passage supplied with lif and scf . pgcs were cloned to third passage supplied with lif and scf on feeder cell . pgcs has the characterization of es - like 采用饲养层培养法，在培养基中添加lif和scf ，分离到了原生生殖细胞，并有2个pgcs集落传至第3代。
It was showed that rh - cm not only could maintain es cells in the undifferentiated state , inhibiting the spontaneous differentiation effectively , but also promote adherence and proliferation of mouse es cells 表明rh - cm能够促进es细胞贴壁增殖和es集落的形成，有效的维持es细胞未分化状态。
It is benefit for blastocysts attached and icm proliferation to supplied with lif and insuine . the es - like cell can be cloned with insulin , and cloned to passage one , nor es - like cell with lif Lif和scf联合使用与对照组相比可提高类es细胞的增殖率( 52 . 9 : 7 . 7 ) ，并且有两个类es集落传至第4代。
The effect of the three culture systems on formation of es colonies at primary passage was not remarkable but significant in . es colonies formation after passage and the highest times of passage 试验中设计的三种培养条件对原代es集落的形成影响不显著，但对传代后的es集落的形成和传代的代次有显著差异。
In this study , the putative eg cell culture media was supplemented with lif , bfgf , scf and igf - 1 . as a result , the ideal dose of lif , bfgf , scf and igf - 1 was : 100iu / ml , 10ng / ml , 10ng / ml , 10ng / ml 而随着培养时间的延长，添加lif 、 bfgf 、 scf 、及igf - 1组表现的优势越来越明显，对促进类eg集落的持续增多效果最佳。
Although the growth factors did have a positive effect on keeping the undifferent state of eg colonies cultured on sto feeders , we can not get eg colonies when pgcs were cultured on 0 . 1 % gelatin in medium supplemented with lif , scf and bfgf 而培养板底只铺有0 . 1的明胶时，即使培养液中加入生长因子也没有形成典型的eg集落。
The researchers also spliced a gene into the virus that makes it produce granulocyte - macrophage colony - stimulating factor , which induces the body ' s immune system to recognize and attack tumors infected by the virus 研究人员还向病毒中插入了一段基因使得受侵细胞分泌粒细胞集落刺激因子，这促进了机体的免疫系统识别和攻击受到病毒感染的肿瘤细胞。
The clonies of es cells were flatter on the mitomycin c - inhibited sto fibroblasts , not easily differentiated from the feeder layer cells surround them , than that on the mef in dmem supplemented 15 % fbs . 5 以sto细胞作饲养层，添加15 fbs ，在倒置显微镜下观察， es - d3细胞不易与周围的sto饲养层细胞区别，形成的集落较平坦，生长状态不如mef饲养层好。
The accine was constructed of lethally irradiated pancreatic cancer cells , engineered to include a granulocyte macrophage colony stimulating factor ( gm - csf ) gene that recruits immune - system cells to seek and destroy microscopic cancers 疫苗是由致命的被照射过的胰腺癌肿瘤细胞株构成，含有粒细胞巨噬细胞集落刺激因子( gm - csf )基因，从而激活免疫系统细胞发现并破坏微小癌细胞灶。
