Telomerase activity was found to be absent in most normal human somatic cells and s . cerevisiae but present in over 90 % of cancer cells . telomerase is closely related with cell growth , proliferation , senescence and carcinogenesis 正常人体细胞(非生殖细胞)及酿酒酵母细胞中端粒酶活性的表达很低，用一般的方法几乎检测不到，在90以上的恶性肿瘤细胞中可检测到端粒酶活性。
Combined with the recent researches , the application of static electricity equipments in fermented food production were summarized , e . g . , wine aging hastening , vinegar and raw soy sauce sterilization and saccharomyces cerevisiae mutation by static electricity , etc 摘要结合近年的研究与探索，对静电设备在酿造品生产中的应用进行了综述，如静电催陈酒类和醋类，生酱油灭菌处理以及用静电诱变酿酒酵母等。
When mms was added directly to s . cerevisiae s288c cell extracts immediately before primer addition , telomerase activity remained unchanged . thus , a direct influence of mms on trap assay does not seem to be involved in telomerase activation observed after drug treatment 如果在加人引物之前将mms直接加在酿酒酵母5288c细胞蛋白提取物zh ，结果显示端粒酶活性没有变化，因此排除了mms对端粒酶的直接激活作用。
Saccharomyces cerevisiae ( s . cerevisiae ) is the one ot the first and well characterized eukaryotic organisms whose complete genome was sequenced . the next chanllenge is to elucidate its gene expression pattern and the functional mechanisms of the gene products 在所有的真核生物中，人们对酿酒酵母( saccharomycescerevisiae )分子遗传学方面的认识最早，最先完成的真核生物基因组序列测定也是酿酒酵母的基因组序列。
A time course experiment of dna - damage and telomerase activity in s . cerevisiae cells treated with 0 . 4mmol / l mms revealed aggravation of dna - damage situation with time prolongation and levels as early as 12h after treatment beginning and increased up to approximately 1 Flmms作用于酿酒酵母5288c细胞不同时间，结果显示随着作用时间的延长， dna的损伤程度加重。同时端粒酶活性于作用12h时就有所提高，当作用72h时几乎达到未用药组的1
The 1 . 488kb dna fragment was sequenced and ligated to pbi121 vector and transformed into otsa deficient of e . coli strains ( ff4169 ) . otsa gene is in charge of trehalose - 6 - phosphate synthesis in e . coli . in growth curve experiment , the transformants that carried the 1 . 488kb dna fragment grew well in minimum medium , which contains 0 . 5mol / l nacl , while control strains could n ' t endure it 提取酿酒酵母的总dna ，以此为模板，采用pcr的方法从酿酒酵母中克隆出了1 . 488kb的海藻糖合成酶基因tps1片段，通过xba和sma双酶切，与同样经过xba和sma双酶切的puc118载体质粒连接，转入大肠杆菌dh5中，通过蓝白斑筛选重组子。
