Protein and nucleic acid in yeast cells were degraded , then the product was refined into nutritional seasoning , which was in step with current tide of nutrition and nature 摘要酵母抽提物亦称酵母味素，是将酵母细胞内蛋白质、核酸等进行生物降解，经加工而成的营养型天然调味料，符合目前“天然、营养、回归自然”之潮流。
The experimental results suggest that the cell disruption ratio can reach 97 . 88 % using enzymolysis and nanometer - crushing machine , and the contents of amino acid in the extractives are also considerably raised 试验结果表明，酵母细胞先经酶解再结合纳米破碎机破碎，可使酵母细胞壁的破碎率达到97 . 88 % ，大幅度提高了抽提物中氨基酸的含量。
For gene expression similarity analysis , we find that there tends to be higher correlations among co - regulated genes when transcription - rate - based correlations are used compared to those based on transcript amounts 我们运用了cho等人的酵母细胞周期mrna量以及wang等人的酵母mrna降解率。在基因相似性研究中，通过数据库， young等人的chipchip数据，我们可以得到共调控基因。
Telomerase activity was found to be absent in most normal human somatic cells and s . cerevisiae but present in over 90 % of cancer cells . telomerase is closely related with cell growth , proliferation , senescence and carcinogenesis 正常人体细胞(非生殖细胞)及酿酒酵母细胞中端粒酶活性的表达很低，用一般的方法几乎检测不到，在90以上的恶性肿瘤细胞中可检测到端粒酶活性。
The labeled cdnas were used to hybridized with the prepared microarray . by using the dna microarray scanner and analysed with the quantarray software package , gene expression profiles of s . cerevisiae in response to heat were studied preliminarily 最后提取热休克前后酵母细胞的总rna ，经逆转录标记合成cdna后与酵母基因表达谱芯片杂交，初步研究了酵母细胞在热应激时基因表达的变化。
By changing pichia pastoris ' s metabolic path rationally , a constitutive recombinant p . pastoris sibas 5071 with riched sam synthetase was obtained on the basis of research of sulfide ' s metabolism in yeast in our laboratory 本实验室在对酵母细胞中含硫物质代谢研究的基础上，通过合理改造pichiapastoris的代谢通路，获得了一株组成型表达sam合成酶2 ( sams2 )的重组p . pastoris菌株sibas5071 。
The yeast two - hybrid system was established . in yeast two - hybrid system , mt - 3 interacts with mt - 3 . a further two - hybrid test also indicated that mt - 3 weakly interacted with its isoform mt - 1 . these primary results suggest that mt - 3 probably trend to homodimerize or heterodimerize in vivo 结果表明： mt - 3与mt - 3之间在酵母细胞内能发生相互作用，进一步用酵母双杂交实验还表明mt - 3与mt - 1间也存在弱相互作用。
The results indicated that the content of cytosolic cam in cells treated with exogenous ca2 + has increased indistinctively , while fluorescence intensity in cells treated with tfp decreased . so we believed that exogenous ca2 + has little effect on the expression of cam 结果发现，经过外钙处理的酵母细胞胞内cam的表达量有所增加，但并不明显，因此认为增加外钙浓度对胞内cam的表达影响不大，但使处于活化态的cam相应增加。
Even so , by truncating hbv pres gene , we finally obtained some useful " " bailors " , either nontoxic or self - activating , and used them to fish dna fragments of hbv pres interacting protein ( s ) from an ad vector constructed human embryonic cdna library 我们通过第回军巨大学硕士学位论文对pres基因分段截短的方法，获得了对酵母细胞即无毒性作用，又没有自激活作用的“诱饵” ，通过它在酵母双杂交系统中筛选构建于ad载体的人胎肝。
Construction and expression of yeast engineering yaccine : s14 / gnsag " as transformed to yeast host straln x33 by means of electroporation after ppiczaa s , . aresag " as l ined by saci enzyme . the single fungus , as choose and dibble inocu1ating in we and am plate , the positive fungus was gro ' ing in rm but not in w , and was 6 inoculated in ypd which included zeocine 500ug / ml and 1000ug / ml . 5 transformers were ampl if ied by pcr , three is same with positive control 选取单个菌落分别点种到删平板和md平板，找出在回d上生长正常， w上生长缓慢或不生长的菌落，即阳性菌落，再以阳性菌落分别涂布zeocine含量500ug加， 1000ug砌l的ypd平板，以高浓度的抗生素筛选高拷贝的酵母工程菌，在含500ug ml高浓度抗生素平板上获得了15个转化子，取其中5个进行pcr扩增，有3个扩增产物与阳性对照相同，说明此酵母细胞中已含有s hbsag融合片段，其中之一命名为p
