Sweetpotato germplasms potted - preservation in intelligent greenhouse in winter in the north is also an important complementary to field - preservation and vitro - preservation 甘薯品种资源冬季智能温室盆栽保存是田间常规薯块保存和试管苗离体保存的重要补充，是我国北方甘薯资源保存新技术、新方法的探讨。
Abstract : this is a review about regeneration of aseptic seedling of panaxquinquefolium l . , methods of the tissue and cell cultures , factors effected on the growth of the cell and the synthesis of ginsenosides 文摘:对西洋参试管苗的再生、组织细胞的几种培养方法、细胞生长及人参皂苷合成的影响因素做了全面的综述。
The paper overviews the formation and characteristics of the technique of rapid propagation of free virus in plant tissue culture , and its application in flower , wood , fruit tree , vegetable . . . etc . , and introduces the main technical link to produce the seedling , including the function and choice of media , the principle and need of donor plants , tame method and transplant request of plant , productive plan of seedling and budge means of cost 摘要综述了植物离体快速繁殖技术和脱毒技术的形成、特点及其在花卉、林木、果树、蔬菜等方面的应用，阐述了利用快繁与脱毒技术生产种苗的主要技术环节，包括培养基的作用和选配要点、外植体选取的原则和快繁与脱毒的不同要求、试管苗驯化的方法与移栽要求、种苗生产计划的制定与成本预算方法。
An in vitro plantlet regeneration system was established from rhizome lumps in polygonatum cyrtonema hua and mannose - binding lectin in cultured rhizome was determined by sds - page and hemagglutination assays and also analysised by rt - pcr and comparison of the nueleotide sequences and amino acid deduced with natural polygonatum cyrtonema hua lectin ii . 利用野生囊丝黄精的幼嫩根状茎作外植体，诱导形成愈伤组织，愈伤组织分化出不定芽，进而产生试管苗，试管苗经移栽试验，成活率为75 ，其外观性状与野生植株无明显差异。
Secondly , we compared and analyzed the growing state , plant height , flower shape and seed setting ability , etc , of m2 tube seedlings and m3 greenhouse plants treated with low - energy n * . we found that the growth rates of m2 tube seedlings and m3 greenhouse plants were lower than that of control and the growth rate decreased with the treated doses from 60x1015 , 40x1015 to 80 x 1015 . vfe also found that many phenotypic variations appeared in m2 tube seedlings , including yellowing , lethality , semi lethality , morphological variation ( such as leaf and flower shape variations , plant height variation , etc ) 最后，对80次剂量处现的拟南芥的mz代试管稍和60次剂员处理的m3代温室茵采用rapd技术对dna的差异进行了分析，检测发现经低能n ”注入处理的材料，其pcr条带缺失或增加，而对照组未发现明显的变化，而且条带的变异率随剂量的升高而上升，从引物的角度看， 80次剂量处理的比代试管苗的多态性表现为67
The phenomenon of vitrification phenomenon is a big obstruction in the propagantion of carnation by tissue culture , the effective way to decrease the vitrification phenomenon is to increase the concentration of sucrose and agar in the medium , to use strong luminosity of 10000 - 20000 lx , and to decrease the concentration of hormone 摘要香石竹试管苗玻璃化现象给试管苗的应用带来困难，采用强光照10000 - 20000lx ，在培养基中提高蔗糖和琼脂浓度，降低激素用量，对克服香石竹试管苗玻璃化有明显效果。
The materials as explant in transformation come from birch leaf , stem segment and leaf stalk , and the spider toxin gene was used as foreign gene for this transformation experiment . it showed that the best explant was the big leaf , on which the transformation frequency was 22 % . by gus detection , there were 43 percent of the plants with kanamycin resistance , and 100 percent of positive result , by pcr amplification , was gotten from random sampling 利用双元载体的根癌农杆菌lba4404菌株( agrobacteriumtumefaciens ) ，含质粒pyhy (目的基因及npt 、 gus基因) ，对白桦试管苗茎段，叶柄，叶片三种外植体进行侵染，结果表明：大叶片生长势强，为转基因的最优外植体，转化率能够达到22 。
