Levels of fasting blood glucose and 24h urinary microcontent of albumin 24 h malb were determined dynamically ; the serum glycosyl hemoglobin hba1c was determined after the last medication ; the ultrastructural changes of kidney were observed by transmission electron microscope ; the expressions of collagen , fibronctin , laminin ln , and the ecm metabolism influencing factors , including mmp2 , tissue inhibitor of metalloproteinase timp2 , transfer growth factor 1 tgf 1 in renal tissue were detected by immunohistological chemistry and image collecting analytical system 动态检测各组大鼠空腹血糖fbg 24h尿微量白蛋白24h malb ，末次给药后测定大鼠血浆糖化血红蛋白hba1c透射电镜观察各组大鼠肾脏超微结构改变，应用免疫组化技术及图像采集分析系统测定各组大鼠肾脏组织中型胶原c纤维连接蛋白fn层粘连蛋白ln的表达，测定影响ecm代谢的基质金属蛋白酶2 mmp2基质金属蛋白酶抑制剂2 timp2及转化生长因子1 tgf 1的表达。
