In the study , respiratory problems and blue ear were not observed on all the pigs after challenged with prrsv strain ch - 1a . but the rectal temperatures of the pigs in the negative control groups ( plers1neo - immunized group and mock group ) were higher than the pigs immunized with the dna vaccines Prrsv的感染一般为亚临床表现，在本试验中，攻毒后所有的小猪都没有出现呼吸困难、耳朵发兰等症状，但空白对照猪和空质粒接种猪的体温要高于基因疫苗免疫猪和灭活苗免疫猪，最高可达40石c 。
67 - 154 . 02 % ( no determinate in paddy field on quaternary red clay ) , respectively . after organic amendments and fertilizers were added to the metsulfuron - methyl - contaminated soils , microbiai biomass c increased by 0 . 23 - 113 . 14 % in paddy field on desalting muddy polder , 0 . 30 - 46 . 48 % in blue clayed paddy , and 1 . 82 - 83 . 76 % in paddy field on quaternary red clay , respectively , microbiai biomass n in correspoading soils by 4 . 27 - 67 . 87 % , 5 . 43 - 58 . 36 % and 5 . 05 - 95 . 40 % , respectively , and microbiai biomass p by 6 . 03 - 139 . 59 % 4 . 09 - 141 . 26 % ( no determinate in paddy field on quaternary red clay ) , respectively ( 4 )添加有机、无机物质后，劳去津除草剂污染的三种土壤中微生物生物量碳、氮随培养时间变化的趋势基本一致，即0 ~ 7d微生物生物碳、氮降低，但第7d时，添加有机、无机物质的处理中微生物生物量碳、氮均高于仅加养去津的处理和空白对照; 7 ~ 14d微生物生物量碳、氮迅速增加; 14 ~ 42d又下降， 42d后变化较小。
We took atrazine and metsulfuron - methyl as examples to evaluate their dynamics of degradation in soils and the responses of microbial biomass after application of organic matters and fertilizers . the objectives are to find out the relationship among of organic matters / fertilizers added , , microbial biomass and herbicides degradation , and for remediation of soils contaminated by herbicides . the main results were summarized as follows : effect of organic matters and fertilizers on microbial biomass in soils contaminated by atrazine / metsulfuron - niethyl herbicides ( l ) the contents of soil microbial biomass c , n and p significantly decreased after application of atrazine at the rate of 22 与空白对照相比，在淡涂泥田中微生物生物量碳、氮、磷分别平均降低了12 . 37 、 11 . 79和11 . 94 ；在青紫泥田中分别平均降低了10 . 70 、 8 . 43和13 . 66 ；在黄筋泥田中分别平均降低了8 . 55 、 11 . 95 (因其酸度较低，微生物生物量磷的测定方法与前两种土壤的不同，所以未测定其中的微生物生物量磷的含量) 。
Injected group , 0 . 1 % saccharin ( 1 . 5 ~ 2ml / rat , in 5min ) intraoral infused group and cta group . the expression of endogenous leucin - enkephalin ( lek ) in the rat brain was observed and 5 parts of the thalamus including laterodorsal thalamic nucleus ( ld ) , lateral part of mediodorsal thalamic nucleus ( mdl ) , ventroposterolateral thalamic nucleus ( vpl ) , ventroposteromedial thalamic nucleus ( vpm ) and reticular thalamic nucleus ( rt ) were comparatively researched before and after the acquisition of cta applying lek - immunocytochemistry . in behavioral experiment , 18 adult male sd rats were divided into normal cta group ( control ) and 2 naloxone i . p 为探讨cta形成过程中enk的作用，本实验用成年雄性sd大鼠35只，分为空白对照组、生理盐水( 2体重)腹腔注射组、 0 . 15mlicl溶液( 2体重)腹腔注射组、 0 . 1糖精溶液口腔灌流组( 1 . 5 - 2ml只， 5min )和cta建立组，采用免疫细胞化学方法，观察了亮脑啡肽( lek )阳性神经元在大鼠脑内的分布情况，并比较了各组大鼠丘脑外侧背核( ld ) 、丘脑内侧背核外侧部( mdl ) 、丘脑腹后外侧核( vpl ) 、丘脑腹后内侧核( vpm )以及丘脑网状核( rt )等5个脑区内lek表达水平的差异；另外将成年雄性sd大鼠18只，分为正常cta建立组以及在cta建立前或cta建立后阿片受体拮抗剂纳洛酮( 2mg kg体重)腹腔注射组，对内源性阿片样物质对于cta建立和保持的影响进行了行为学研究。
Elisa results showed that the translation level of il - 1 and tnf - a detected in culture supernatant of oligochitosan contrast group were as 2 . 4 times and 2 times as the level of blank contrast group ( p < 0 . 01 ) , and the translation level of il - 1 and tnf - a genes of experiment group were as 4 Elisa实验结果显示，两种细胞因子分泌也显著增多，加糖对照组il lp和tnf a分泌量分别为空白对照组的2 4倍和2倍b 0 of人实验组几刀p和tnf a分泌量分别为空白对照组的4
