Analyse the research of distributing of microbe community and the tendency of the change , disscuss the principle and traits of denaturing gradient gel electrophoresisand terminal restriction fragment length polymorphism , to research the law of change that the microbe community have in composting process , we can get effective and rapid information to filtrate the microorganism during composting process , then accelerate the development of compost technology 摘要对堆肥微生物种群分布及其动态变化的研究进行了分析，论述了分子生物技术中的变性梯度凝胶电泳和末端标记限制性片段长度多态性的原理和特点，以及用于研究堆肥微生物的群落结构演变规律，为分析和筛选堆肥中的微生物提供更加有效、快速的信息，促进堆肥技术的发展。
In this study , the whole e2 genes of two strains of classical swine fever virus ( csfv ) isolated from guangxi ( gx ) were amplified by reverse transcriptase polymerse chain reaction ( rt - pcr ) method and seqenced . the e2 gene fragments of csfv were 1090 base pair in length and encoded 364 amino acid residues 研究采用反转录?聚合酶链式反应( rt ? pcr )技术对两株分别从柳州( gxlz )和南宁( gxnn )分离的广西流行猪瘟病毒( classicalswinefevervirus ， csfv )进行e _ 2全基因的扩增、克隆和测序。扩增片段长度为1090bp ，编码364个氨基酸残基。
The dissertation consists of five chapters : in chapter one , the recent progress in molecular approaches in systematic studies of macroalgae e . g . dna extraction , restriction endonuclease fragment length polymorphisms ( rflps ) , random amplified polymorphic dna ( rapd ) , gene sequencing , intersimple sequence jepeats ( issr ) , amplified fragment length polymorphisms ( aflp ) and single strand . conformation polymorphisms ( sscp ) were reviewed 本论文由五部分组成：在第一部分，综述了大型海藻dna的提取、限制性片段长度多态性( rflps ) 、随机扩增多态性dna ( rapd ) 、核酸序列分析、扩增片段长度多态( aflp ) 、单链构象多态( sscp )等分子手段在大型海藻系统学研究中应用的一些进展。
Objective : as one of the widest length polymorphic genetic markers , short tandem repeat ( str ) , also named microsatellite dna or simple sequence repeats ( ssrs ) , are currently used in forensic hematogenetics . the short tandem repeat loci are widely dispersed in the human genome and have high sensitivity and discrimination power 目的:短串联重复序列( shorttandemrepeats , str ) ,又称微卫星dna或简单重复序列( simplesequencerepeats , ssrs ) ,是目前在法医物证学中应用最广泛的片段长度多态性遗传标记。
