The purpose of my work was to clone and express of mouse endostatin gene , we construct a temperature - sensitive expression system : pbv220 - endostatin . the main work was as following : 1 本文进行小鼠内皮抑素基因的克隆及表达，基因工程方法构建了鼠内皮抑素的温敏型表达载体pbv220 - endostatin ，为此作了以下工作： 1
The preparation of copper nanoparticles / paraffin thermosensitive composite materials is discussed firstly in the dissertation . the copper nanoparticles / paraffin composite powders are prepared by high energy milling 本文首先研究了纳米铜粒石蜡温敏复合材料的制备方法，利用高能球磨法制备了纳米铜粒石蜡复合颗粒。
The t - r characteristics may originate from the total function of volume expansion and temperature susceptibility of asphalt , the deference of volume expansion coefficient with heat between asphalt and graphite , and internal stress 而温敏产生的原因则为沥青的体积膨胀、石墨和沥青的热膨胀系数差异、内部应力和沥青温度敏感性共同作用的结果。
According to the stress - strain condition at different parts , cfrc is laid selectively , not only the higher compression sensitivity can be attained , but also the interaction between compression sensibility and temperature sensibility of cfrc is divided 根据构件各部位的应力应变状况及cfrc的功能特性，有选择地敷设cfrc ，采用直流探针压降法测试，既获得了极高的压敏灵敏度，又实现了cfrc压敏性和温敏性两种效应的解耦。
Using these vectors , expression of the pks gene was achieved both in streptomyces lividans and in e . coli in a heat - dependent manner , suggesting that the lambda promoter and temperature - sensitive lambda represser functioned in s . lividans as well as in e . coli 利用这些质粒在变铅青链霉菌和大肠杆菌中均表达出pks蛋白，两种宿主中的表达都是热依赖的。暗示噬菌体启动子和温敏型阻遏物在变铅青链霉菌和大肠杆菌中都具有功能。
The factors such as the forming technology , the milling time and the mass ration of copper and paraffin in the composite materials which affect the thermal sensitivity , the thermal expansion and the thermal stability of the composite materials are investigated 通过压力成型的方法获得纳米铜粒石蜡温敏复合材料。研究了复合材料不同成型工艺、球磨时间以及复合材料中所含铜粉和石蜡不同质量配比对复合材料热敏性、热膨胀性和热稳定性的影响。
Ansys finite element analysis software is applied for simulating the temperature field of the paraffin and the copper . the time needed of the cylindrical paraffin and the cylindrical copper reaching the thermal equilibrium is calculated respectively , and compared with the experimental data 使用ansys有限元分析软件，分别对石蜡和铜受热时内部温度场分布进行了模拟，理论计算出圆柱状石蜡和圆柱状铜整体达到热平衡状态所需的时间，并且和实验中所测纳米铜粒石蜡温敏复合材料达到热平衡状态所需的加热时间进行了对比分析。
The result shows are as following , first , using a . c . impedance measurement to measure the compression sensibility of cfrc can eliminate the polarization effect at the electrode of cfrc specimen and the thermoelectric force of the electrode effectively ; the thermo sensitive effect can be avoided by using little electric current , so the a . c 结果表明：采用交流阻抗法测试cfrc试块的压敏效应可以有效地消除cfrc试块的电极处的极化效应和电极界面的热电动势；采用小电流供电可避免温敏效应，因此交流阻抗法要比直流电阻法稳定性好。
A 1 . 7kb fragment encoding ge of prv fa strain was obtained by pcr from plasmid ppge templated using a pair of the designed primers containing ecori and bamhi ' sites . the ge gene fragment cutted with ecori and bamhi was inserted into the expression plasmid pbv220 including these two endonuclease sites for constructing the recombinant plasmid pbvge . strain dh5a of e . coli contain pbvge was induced at 42 for 4 - 6hr after incubation with vigorous shaking at 30 for 3hr or so 以质粒ppgedna为模板， pcr扩增出1 . 7kb的ge基因完整片段，将扩增产物以ecori和bamhi双酶切后，插入原核表达载体pbv220的p _ rp _ l启动子下游的ecori和bamhi位点间，得到重组表达质粒pbvge ，转化了pbvge的大肠杆菌dh5a经温敏诱导表达后，用sds - page和western - blot ，以及琼脂双扩散来检测，结果表明prvfa株ge基因在原核载体上得到高效表达，表达产物约占总蛋白的17 。
So the biomaterials which are used in treatment , particularly the materials which use for actifical machines are in the situation being used only , faring away from application . at the beginning of 1990s , several study groups include matsuda et . al firstly pointed out the surface modification of medical polymer , materials - photo - chemical immobilization , which was dramatically different from any other surface modification process available for medical materials 在第一章中，首次采用光固定法将合成的温敏性能显著， nipaam与aa共聚物( pia ， lcst为38 . 5 )的叠氮苯基衍生物( azphpia ， lcst下降为21 . 5 )区域接枝到组织培养用聚苯乙烯( ps )膜上，制成具有区域温敏性能的温敏生物材料。
