In our experiments , we transformed the grifola frondos ( bacidiomycete ) trehalose synthase gene ( tsase ) into tobacco to make further study to verify the function of the gene 本研究利用担子菌灰树花海藻糖合酶基因( tsase )遗传转化烟草，以进一步验证该基因的功能。
Methods : using mouse which were fed by different drinks ( water , sucrose water and trehalose water ) respectively as testing animals which were exposed to 30 habitat 方法：以小鼠为模型，考察不同成分饮用水（水、蔗糖和海藻糖）饲喂的小鼠热暴露的存活时间和衰竭游泳时间。
In yeast , the synthesis of trehalose is catalyzed by trehalose - 6 - phosphate synthase / phosphatase complex , which is encoded by four genes : tpsl , tps2 , tps3 and tsl1 酵母细胞中的海藻糖由tps1 、 tps2 ， tps3和tsl1四个基因负责合成，其编码的多肽组成一个海藻糖合酶复合体。
Trehalose , a non - deoxidize disaccharide , serves as a protectant of enzymes and membranes in many microorganism , algae and lower invertebrate animals against various stress 海藻糖是由两个葡萄糖分子以、 1 1糖苷键结合的非还原性双糖，对许多生物的抗逆耐受性起着重要作用。
Through the test , the strain of y7 , which had the highest content of trehalose and strong tolerance to sodium chloride and acetic acid , was defined as a parental strain 综合耐性实验和海藻糖积累实验的结果，确定了海藻糖含量高，渗透压和乙酸耐性最强的y7菌株为本研究出发菌株。
Meanwhile , the inhibition of trehalose for the retrogradation could be more apparent during the frequent courses of freezing and thawing , according to the analysis of the preservation condition 同时，通过对保存条件的分析，发现海藻糖在频繁的冻融过程中更能体现出抑制回生的效果。
At this point , continual drying induces a phase change of the sugar to a glass state so that the protein molecules are surrounded by trehalose crystals , preserving them from thermal energy ( 46 , 47 ) 这时候，连续干燥引起糖的相变，变成玻璃状态，这就使得蛋白分子被海藻糖包围，从而避免受热。
It was found that replacing part of granulated sugar in the complex solution of granulated sugar and sticky rice starch with trehalose could be effectively stay the course of retrogradation 研究发现，在含有糯米粉、白砂糖的复合体系溶液中，用海藻糖替代部分白砂糖，能有效地延缓回生的进程。
The tpsl gene was introguced into immature embryo cells of 12 - 16 days wheat cultivars wen . 6 via gene gun delivery technique . resistant calli were selected on medium containing hygromin ( 40 - 60mg / l ) 用基因枪将海藻糖- 6 -磷酸合成酶基因( tps1 )导入小麦栽培品种温6的12 - 16日龄的幼胚细胞中。
Trehalose is accumulated at the end of the reproductive stages and under extreme environmental conditions . because the trehalose is intracellular product , its productivity is limited 在酵母生长后期或在不良环境的条件下可积累大量海藻糖，但由于是胞内产物，积累量有限，大大影响了产量的提高。
