The reason for this is unknown at the present . possibly , cg - rich and at - rich regions in amphioxus dna are not as " rich " as those in the vertebrate dna , that is , amphioxus dna ha 虽然nogusa ( 1957 )认为b . belcheri存在xy型的性染色体，至于青岛文昌鱼的性染色体是xy型或zw型尚待深入研究。
So , the cloning of amphioxus masp gene is very important in the development of masp gene and the immune system , even the origin of the vertebrates 因此，文昌鱼masp基因的克隆对于研究中国海洋人学硕几l . .学位论文功asp基因乃至免疫系统的进化来说是非常重要的，同时对研究脊椎动物的起源也具有十分重要的理论意义。
The hemolytic activity was mg2 + - dependent and heat - sensitive , and was abrogated by treatment with rabbit anti - human c3 serum , zymosan , methylamine , hydrazine , and phenylmethylenesulfonyl fluoride ( pmsf ) 文昌鱼体液对兔血红细胞的溶血活性在受到酵母聚糖、甲胺、肼、 pmsf 、 edta 、兔抗人补体3抗血清处理时，溶血活性消失。
In the present study , a cdna expression library was constructed according to smart cdna library construction kit user manual from the adult amphioxus guts using pcdna3 eukaryotic expression vector 本文应用smart ~ ( tm ) cdnalibraryconstructionkit的方法，以成体文昌鱼肠为研究材料，构建了以真核表达载体pcdna3为基础的cdna表达文库。
Functional study on these novel genes is an on - going task in our laboratory . amphioxus amphihmgb encoding a hign - mobility - group ( hmg ) protein was identified from the gut cdna library of the amphioxus b . belcheri tsingtauense 文昌鱼肠cdna表达文库的成功构建，为从分子生物学水平研究文昌鱼的进化与发育奠定了基础，也是分离新基因的重要来源。
Rapd analysis was applied to study genetic diversity of amphioxus ( branchiostoma belcheri tsingtaoense ) in the test . total twenty - two samples , which are eleven males and eleven females were collected for use 本实验首次采用随机扩增多态dna ( randomamplifiedpolymorphicdna ， rapd )技术对青岛文昌鱼该群体雌雄各11条个体共22个样本进行遗传多样性检测。
The counting of 150 well - spread samples revealed that the number of the chromosomes ranged from 18 to 38 , but the modal number of the chromosome was 36 , which was in proportion to 66 % 计数150个染色体分散好的中期相，发现染色体数目的变化范围是18至38条，长度为1 . 4至3 . 7 m ，染色体的众数是36 ，占150个中期相的66 ，说明青岛文昌鱼染色体的数目是36 。
Sequence alignment shows that the fragment of pcr product showed some identities to the masp gene of xenopus laevis , branchiostoma belcheri , the trypsin gene in litopenaeus vannamei and the serine protease gene in aurelia aurita Pcr反应产物基因片段大小为630bp ，序列比较结果表明， pcr克隆产物与爪蟾、文昌鱼的masp基因，虾、水母等的胰蛋白酶基因和丝氨酸蛋白酶基因都有一定的同源性。
The complementarity of g - and r - band patterns was evaluated by comparing the features of each positive and negative chromosomal g - and r - band . it was found that the complementarity is only illustrated to some extent by the parts of some chromosomes 精确计算和比较每条染色体的g 、 r阳性和阴性带纹特征，发现有些染色体呈一定的互补性，但这种互补性在文昌鱼染色体上较低，大约只有38 %的部位存在互补现象。
In addition , titration by turbidimetric immunoassay ( tia ) using sheep anti - human c3 serum revealed that amphioxus humoral fluid contained c3 - like component , and its concentration is about 1 . 17 mg / ml , which is comparable to c3 concentration in human or dog sera 此外，本实验还运用免疫比浊法以绵羊抗人的补体3的抗血清检测了文昌鱼体液中的补体3浓度为1 . 17mg ml 。此浓度和人血清及狗血清中的c3浓度基本相当。
