The biological activity of expressed products were examined with thiazoyl blue tetrazolium bromide ( mtt ) method , and the myoblast as target cell . it concluded that the biological activity of des ( l - 3 ) igf - l protein was 1 . 5 to 4 times higher than that of igf - 1 form 为了检测其生物活性，用重组人完整型igf - 1蛋白和截短型igf - 1蛋白分别刺激c2c12成肌细胞，然后用mtt （噻唑兰）法比较了这两种蛋白对c2c12成肌细胞的促增殖能力。
Even so , by truncating hbv pres gene , we finally obtained some useful " " bailors " , either nontoxic or self - activating , and used them to fish dna fragments of hbv pres interacting protein ( s ) from an ad vector constructed human embryonic cdna library 我们通过第回军巨大学硕士学位论文对pres基因分段截短的方法，获得了对酵母细胞即无毒性作用，又没有自激活作用的“诱饵” ，通过它在酵母双杂交系统中筛选构建于ad载体的人胎肝。
Conclusions : prokaryotic and eukaryocytic expression plasmids of the shortened hepatitis b surface antigen were successfully constracted , and the target proteins expressed by iptg induced in escherichia coli . as well as in eukaryocyte ( hepg2 and cos - 7 ) , then their antigenity were detected 结论：截短的乙型肝炎表面抗原分子的原核和真核表达’重组质粒成功被构建及分别在人肠杆菌efl得到诱导表达和存贞核细胞ifj表达，并检测剑其表达产物的抗原特性。
The finite precision effect brought by the digital realization of cs and the deterioration of the sequence ' s properties caused by interception have been studied . moreover , after analyzing the effect of the existing ways to improve the performance of cs , especially the perturbation , the effectness of a kind of chaos sequence generator by means of software high - precision computation ( shpc ) has been disscussed 在数字混沌序列方面，本文验证了混沌扩频序列由于数字化所产生的有限精度效应、截短所造成的性能下降，同时对目前的改进方法，主要是加扰法，进行效果分析，并对利用软件高精度计算实现数字混沌序列产生器的方法进行了探讨。
Then based on the analysis of the constructions of wh , go and ls sequences , the corresponding correlation properties are derived and methods of selecting subsets with good periodic mean - square cross - correlation property or aperiodic mean - square correlation property from the wh set and go sequence set whose go zone z = l are investigated . furthermore , the methods of truncating the go sequences to obtain the quasi - orthogonal ( qo ) set whose family size m is larger than the length l and the quasi - generalized - orthogonal ( qgo ) set whose size m is larger than li2z are discussed , together with the numerical results of the correlation properties 基于这些结果，探讨了如何从wh序列集和广义正交区z = 1的go序列集中，分别按照周期互相关均方特性最优和非周期相关均方特性最优的准则选择性能较好的序列子集；同时讨论了将广义正交序列截短来获得准正交( qo )序列集和广义准正交序列集( gqo )的方法，并给出了这一类序列相关特性的数值计算结果。
Thereafter , the applications of go sequences in single - cell s - cdma systems are discussed in this thesis . in additive white gaussion noise ( awgn ) channel , interference components in s - cdma systems accommodating more users than the spreading factor is analyzed based on the theoretical bound of the periodic correlation mean square property . then two interference - suppressed s - cdma system models of augmented capacity are proposed , along with the analysis and simulation results 在加性白高斯噪声( awgn )信道条件下，以序列集周期相关均方特性理论界为基础，对序列个数大于系统扩频因子的s - cdma系统干扰组成进行了分析，并基于截短wh序列提出了两种高容量低干扰s - cdma系统模型，给出了分析和仿真结果。
Full - length or truncated cdna was subcloned into prokaryotic expression vector pet30a and expression induced in e . coli bl21 ( de3 ) . no squalene synthase polypeptide of expected molecular mass was observed in e . coli containing the putative full - length squalene synthase cdna , however , overexpression in e . coli was achieved by truncating 30 hydrophobic amino acids at the carboxy terminus 但在含有全长的鲨烯合酶cdna的大肠杆菌中并没有观察到预期大小的鲨烯合酶表达，而c末端截短30个疏水氨基酸的鲨烯合酶可在大肠杆菌中过量表达。
In this article , the author amplified dna sequences encoding the mature peptides of human intact and truncated igf - 1 gene from human genomic dna by the new way of alternative pcr established and named as exons - piecing together method by this laboratory . the igf - 1 and des ( l - 3 ) igf - l gene were cloned in pgem - t vector and then subcloned in expression plasmid pgex - 3x . two recombinant constructs known as pgex - 3x - igf - l and pgex - 3x - des ( l - 3 ) igf - l were transformed to e . coli dh5 a respectively 我们利用外显子拼接法从人的基因组dna中扩增了编码完整型及截短型人igf - 1基因成熟肽的dna片段，并将其克隆至pgem - t载体中；经测序证明正确后，又构建了表达载体pgex - 3x - igf - 1及pgex - 3x - des （ 1 - 3 ） igf - 1 ，在大肠杆菌中进行了表达研究。
Human insulin - like growth factor - 1 ( igf - 1 ) is a basic polypeptide containing 70 amino acid residues . its truncated form , i . e . des ( l - 3 ) igf - 1 , is three residues shorter than the intact form at n - terminal . igf - 1 has many biological actions such as mitogenic , metabolic effects and others <中文摘要> =人胰岛素样生长因子1 （ insulin - likegrowthfactori ， igf - 1 ）是一个由70个氨基酸组成的碱性多肽，其截短型比完整型的n端少了三个氨基酸（甘氨酸、脯氨酸、谷氨酸） 。
