The main works already done include the effects and mechanism of stimulation of renzhong on respiration and blood pressure , the electrophysiological and morphological properties of the respiratory neurons in the medulla oblongata of rat , the roles of the medullary respiratory neurons in the regulation of respiration and respiration - related activities , the relation of the nucleus paragigantocellularis lateralis with the control of respiratory rhythm , the roles of the neurons of pre - botzinger complex in the control of respiration and their neurochemical properties , and the establishment of a simple and effective technique of decerebrating rat 已完成的系列研究工作主要有：人中的呼吸和血压效应及其机制大鼠延髓呼吸神经元的电生理和形态学特点细胞内研究延髓呼吸神经元在呼吸和呼吸相关活动调节中的作用旁巨细胞外侧核与呼吸节律前包钦格复合体神经元的神经化学特性和在呼吸调控中的作用建立了一种简便有效的去大鼠大脑方法。正在进行的科研工作主要有：
Fos + / th + / gfap + and fos + / vp + / gfap + triple labeled n - asc could be found in the mvz , pvn and son respectively ; ( 2 ) under electronic microscope , the astrocytic processes connected closely with the dendrites or axons of the neurons , where the bilateral membranes became thick . we call transiently it electron - dense areas ( edas ) . the number of edas increased remarkably following hyperosmotic stimulation ; ( 3 ) when trace retrogradely , wga - hrp was microinjected into the unilateral son , pvn or nucleus of solitary tract ( nts ) respectively using the stereotaxic method , the n - ascs formed by the neurons triple - labeled with hrp / fos / th ( or vp ) and astrocytes labeled with gfap could be found in the mvz , son and pvn respectively ; ( 4 ) after being treated with heperosmotic nacl solution , intracellular calcium concentration in cultured hypothamic neurons and astrocytes increased and then decreased 脑内gfap阳性结构也明显增多，其分布与fos阳性细胞分布基本一致，表现为胞体肥大、突起粗长； ast紧密包绕在神经元周围形成神经元- ast复合体( n - asc ) ；在mvz 、 pvn和son三重免疫组化染色切片上可见到fos + th + gfap +第四军医大学博士学位论文和fos vp gfap三重标记asc ； （ 2 ）免疫电镜下son内星型胶质细胞突起与神经元树突或轴突之间接触部位出现增厚的膜结构一电于致密区（ edas ） ，高渗刺激后数量明显增多： （ 3 ）将们个mp注入大鼠一侧n卜、卜卜或孤束核（ ws ） ，分别在延髓内脏带（ mvz ） 、 so和pvn内出现fos hrp th 、 fos hrp八p三重标记神经元和gfap阳性标记ast形成的n asc ； （ 4 ）高渗刺激使培养神经元和ast内钙水平先升高后降低，最后维持在比高渗刺激前稍高的静息钙水平上。
Collection and preservation of samples : as soon as the three vital signs disappeared , the dogs were anatomized , and the heart , liver , kidney , spleen , lung , brain , muscle in the injection location and no injection location , the heart blood , urine , bile , cerebrospinal fluid ( csf ) in the lateral ventricle and spinal subarachnoid space , spinal cord ( medulla oblongata , cervical cord , the upper beast spinal cord , breast spinal cord and waist spinal cord ) were taken out , some of which were preserved at - 20 for qualitative and quantitative analysis , and the others were fixed with 4 % formaldehyde for the pathology observation 3 、样品采集：当心电、血压和呼吸全部消失时，迅速解剖动物，采取心脏、肝脏、肾脏、脾脏、肺脏、大脑、注射部位肌肉、注射部位20cm以外肌肉、心血、尿液、胆汁、侧山西医科大学硕士学位论文脑室脑脊液、脊髓腔脑脊液和不同节段的脊髓(包括延髓、颈髓、上胸部脊髓、胸部脊髓和腰部脊髓)等组织，冷冻保存。 4 、病理观察:采取心脏、肝脏、 ’肾脏、脾脏、肺脏、大脑、脊髓等组织， 4 %甲醛固定，石蜡包埋，切片， he染色，光镜观察。
Moreover , human ghrelin receptor exhibits a remarkable 50 % overall identity with human motilin receptor , therefore it was named as " raotilin - related peptide " . anatomical evidence showed that there are ascending and descending neuronal projections between pvn , bma and lower brain stem ( nts , dmx ) , which participate in the regulation of gastric acid secretion and gastrointestinal motility . moreover , pvn is the center of appetite regulatory web Pvn与低位脑干(延髓迷走复合体)及边缘系统之间有密切的纤维联系，且含有多种神经肽，参与胃酸分泌，胃肠活动等多种生理机能的调节，是影响消化道功能与摄食行为的重要中枢部分， pvn内胃动素可促进胃排空和摄食，而新发现的ghrelin /胃动素相关肽是否参与此核区对胃运动的调节机制及其可能的神经通路尚不明了。
The present studies aimed to find the responses of neurons and glias in the target and other brain areas of the linear accelerator - irradiated rats . in the first experiment , time - dependent neurons changes in the brain after hemispheric irradiation at an single - fraction maximal dose of 20 gy were observed by investigating the expression of an immediate early gene , fos protein . the results shown : compared with that of the un - radiated rats , the expression of fos protein in the irradiated brain decreased distinctly 24 hours and 1 week after irradiation 首先，利用即刻早期基因fos的表达，做了同一照射剂量( 20gy )照射大鼠半脑后不同存活期间( 1d ， 7d ， 14d ， 2ld ， 28d )大鼠全脑神经元动态变化的观察，发现：受照射后1d 、 7d大鼠脑内各部位fos蛋白表达均明显减少，随着时间的延长，其fos免疫反应性细胞数量逐渐增加，照射后28d ，延髓、第四军医大学硕士学位论文脑桥内hs免疫阳性细胞数量恢复并超过正常对照组水平，但中脑、间脑及端脑内未恢复到正常对照组水平。
