Two stage embryos were observed their effect on production of chimera . the result shows that there is a significant different ( 0 . 01 < p < 0 . 05 ) between blastocysts ( including early blastocysts and morulae ) and expanded blastocysts 结果表明,囊胚、早囊及桑椹胚同扩张囊胚两种注射胚胎制作嵌合体所产生的结果差异显著( 0 . 01 p 0 . 05 ) 。
Over this period cells gradually came to live free from bodies , then experiments conjured them bit by bit into freezable , mass - produced objects and eventually into chimeras with parts from more than one species 在这期间内,细胞逐渐能脱离身体存活,然后实验一点点的将其变成可冷冻、大量生产的物质,最终成为由来自不止一个物种的部分组成的嵌合体。
Moreover , chimeras when injecting of the cultivated cells into receptor ' s x stage blastdermal also showed that the cells were some pluripotent . these study may be useful to the production of transgenic chickens by genomic modification 本研究分离得到的类eg细胞经形态学鉴定、 pas染色、体外分化实验和嵌合体鸡的制作等实验证明其具有胚胎干细胞的诸多类似特性。
The researchers took a two - step approach : first they injected uniparental es cells into wild - type blastocyts to generate chimeric animals ; then they harested these chimeric fetal liers for transplant into lethally irradiated hosts 研究人员采取了两步骤的方法:首先,他们将单系胚胎干细胞注入野生型的胚细胞培育嵌合体动物;然后收集嵌合体胚胎肝脏移植入受致命性辐射照射的宿主。
The research of the renaturation and biological activity of the chimeras confirmed the active sites of hwtx - i and mviia further . it also indicates that the structure of ick is stable and is an ideal model molecule for protein engineering 通过对嵌合体复性和活性情况的研究,进一步证实了hwrtx一i和mvlla的活性位点;并且表明抑制剂胧氨酸结模体( ick )的结构确实是相当稳定的,是一种进行蛋白质工程的较理想的模型
After renaturation , the biological activity of mviia is nearly 100 % of that of natural one ; the activity of hm - 1227 is less than t | iat of hwtx - i ; however , hm - xw cannot block the neuromuscul ar transmission in an isolated mouse phrenic nerve - diaphragm preparation 复性后的mv a摘要生物学活性接近天然mvlla的100 % ;嵌合体hm一1227的活性小于hwtx一i的15 % ;而嵌合体fd以一xw不能阻断小鼠隔神经一隔肌的接头传递。
Hfea deferred a decision on other types of human - animal embryos , such as what are known as " true hybrids " ? created by the fusion of a human sperm and an animal egg ? and so - called " human chimeras , " where human cells are injected into animal embryos Hfea延缓了针对另外一种类型的人-动物胚胎的决定,例如已为我们熟悉的“真杂交体” ? ?通过融合人精子和动物卵细胞制造的杂交体? ?因此被成为“人嵌合体” :人细胞被注射到动物胚胎中。
Second , balb / c mice were conditioned with 9 . 0gy lethal tbi and divided into two groups randomly . group a were injected with bone marrow cells 4x107 and speen cells 2107 from chimeric rats , and group b injected with bone marrow cells 4xl07 and speen cells 2xl07 from normal rats 第二步, balb c小鼠经至死剂量全身照射后随机分为两组, a组输入嵌合体大鼠的骨髓细胞和脾细胞, b组输入正常大鼠的骨髓细胞和脾细胞,比较gvhd发生情况。
One chimaeric mouse was produced by injection of es cells into blastocyst of c57bl / 6j . the chimaeric rate of fur colour was about 15 % . fl generations of germ - transmission derived from es cell were not obtained when chimaeric mouse mated with kunming albino mice and c57bl / 6j mice 将胚胎干细胞注射到c57bl 6j小鼠的囊胚腔内,移植后产生了一只wk2 -胚胎干细胞的雄性嵌合体鼠,毛色嵌合率为15左右,成熟后分别与c56bl 6j和昆明白雌鼠交配,均未得到来自es细胞的后代。
164 microinjected embryos and 121 noninjected embryos were transferred to 14 recipient gilts . of this , 159 microinjected and noninjected embryos ( blastocyst , early blastocyst and morula ) was transferred to 6 recipient gilts , three pigs are pregnant ( one gilt delivered 8 piglets , two are skin pigment chimera ) , and pregnancy and chimera rate is 17 % ( l / 6 ) . 126 microinjected and nonmicroinjected expanded blastocysts oestrused on time , pregnancy and chimera rate is 0 ( 0 / 5 ) 其中, 159枚囊胚、早囊及桑椹胚混合胚移植到6头受体, 3头妊娠(其中一头母猪产仔8头,两头为皮肤色素嵌合体) ,妊娠嵌合率为17 % ( 1 / 6 ) ; 126枚扩张混合囊胚移植到5头受体均按时返情,妊娠嵌合率为0 ( 0 / 5 ) 。