Thanks to the generous financial support from agilent technologies , the nature conservancy ( tnc ) china program is pleased to invite applications and nominations for an exciting tnc / agilent senior conservation fellowship program 感谢安捷伦慷慨的财政支持，美国大自然保护协会（ tnc ）中国项目现在欢迎有关专家申请或提名申请我们的高级环境保护人员研究基金项目。
The author reviewed the detection measures of prunus necrotic ringspot virus , and related the research progression of the pathogen detection technology inside and outside . the template amplication technology include pcr assays ^ nasba and so on . the cdna and crna probe which labeled with the isotope % biotin or dig , the offset probe and the peptide probe can be applied to magnify the signal . pcr - gene scan assays and pcr agilent chip chamber combine the template amplication and the signal magnification 本文回顾了李坏死环斑病毒的检测方法，较全面地评述了国内外病原物检测技术研究进展：在模板的扩增有各种pcr技术、 nasba技术；在信号的放大有同位素、生物素或dig标记的cdna和crna探针，分支探针和肽核酸探针；模板扩增和信号放大相结合的有pcr -基因扫描技术、 pcr安捷伦芯片实验室技术；模板扩增和杂交以及信号放大相结合的有pcr - elisa技术、实时荧光pcr技术、生物芯片技术。
