And without the administration ' s goal of a “ bullet - proof agreement ” that business lobbyists can enthusiastically sell to a republican congress , the whole process will end up in partisan acrimony that could harm relations with china for years 没有商界院外活动集团成员热情的劝说共和党国会采纳政府目标中的防弹性（保护性）协议，那么整个过程将会以党派之间的尖刻的争吵而结束，这会影响以后多年和中国的关系。
The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3 , then the vector was transferred into pachia pastoris gs115 strain . the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation . sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku 将缺失型pap基因克隆于酵母分泌型表达载体ppicgk构成重组载体，然后导入毕赤酵母（ p8chianastoris ）菌株gslls细胞中，在甲醇的诱导下，经过酵母高密度发酵进行pap的表达，经sds page分析，结果表明，在培养基上清液中含有一明显的特异性蛋臼条带，大小为34ku ，经western blotting分析，该蛋白与法国pap抗血清有特异性反应，体外活性检测表明该蛋白对tmv的侵染性具有高度的抑制性，说明该pap基因在毕赤酵母gs中也得到了正确表达。
A 24 hours continuous research of shinisaurus crocodilurus ' s movement . we add up the shinisaurus crocodilurus ' s outing activities according to different time of a day . we also add up the influence of different seasons and climate on the number of shinisaurus crocodilurus ahl . 每天24小时连续观察鳄蜥的活动情况，统计一天中不同时间内出洞外活动的鳄蜥数量以及不同季节、气候条件下的数量变化，以观察到的不同时间内活动个体的多少来表示鳄蜥的活动节律变化。
