Finally , a combined scheme was proposed to optimize the refolding process , this work demonstrates the feasibility of applying fed - batch and / or step - addition operation schemes to the renaturation of enzyme activity 最后本研究中，结合馈料式与间歇式复性法之操作策略，可更有效率地提高其复性效率。
This paper describe the formation , isolation , denaturation and renaturation of recombinant proteins as inclusion bodies in e . coli , and summarize the most efficient ways to refold recombinant proteins 摘要描述了大肠杆菌异源重组蛋白质的形成、制备、变性和复性，综述了国内外变性、复性的有效方法。
Then the total 25 engineered strains were cultured and induced in the respective conditions . the results show that the target products expressed in 3 strains of them are partly soluble and active , whils 11bl21 ( 0e3 )表达的包涵体蛋白进行了变性、复性研究，部分获得有活性的d一海因酶，酶活力约为0 . 90u / mgo
The aggregate of egg white lysozyme molecules formed during the refolding procedure of denatured - reduced egg white lysozymes was analyzed by protein electrophoreses and high - performance size - exclusion chromatography 本文利用蛋白电泳和高效凝胶排阻层析法分析了还原脉变性蛋白溶菌酶稀释复性过程中的集聚体。
The aggregate of egg white lysozyme molecules formed during the refolding procedure of denatured - reduced egg white lysozymes was analyzed by protein electrophoreses and high - performance size - exclusion chromatography 摘要本文利用蛋白电泳和高效凝胶排阻层析法分析了还原脉变性蛋白溶菌酶稀释复性过程中的集聚体。
At first , the systematic studies was made on the mechanisms and optimal conditions of denaturation and renaturation of mviia which lay the groundwork of renaturation of chimera of hwtx - i 先利用合成的mviia为模式肽，系统的研究了此毒素的还原氧化复性机制和最佳的复性条件，为hwtx -嵌合体的氧化复性奠定基础。
Our research shows that the fed - batch and the step - addition schemes overcame the aggregation formation and favored the refolding step at higher feeding concentrations in comparison with the typical dilution method 由实验结果显示，馈料式与间歇式复性法相较于稀释法已有效地避免聚集体之生成及提高最终的操作浓度。
4 the primary result of inhibition experiment of blood vessel proliferation in the chicken chorioallantoic membrane demonstrate that r hpf4 prepared by our methods has bioactivity like wide hpf4 4鸡胚绒毛尿素膜血管生成抑制实验测定复性后的rhpf4的生物学活性，初步结果显示： rhpf4具有野生蛋白抑制血管生成的活性。
By the use of cirular dichroism and fluorescence techonologies to predict the secondary structure of protein , to determine the secondary structure of a protein , and to explore the unfold - refold mechanism of a protein 运用圆二色谱和荧光技术分析预测蛋白质二级结构，研究蛋白质的稳定性与蛋白质的变性复性动力学机制。
The documented evidence that the process , operated within established parameters , can perform effectively and reproducibly to produce a medicinal product meeting its predetermined specifications and quality attributes 用文件证明该工艺，在规定参数下操作，可以有效并重复性良好的运行，并可以生产出符合预期标准和质量要求的产品。
