After sonification , the precipitate was washed repeatedly by ionic and non - ionic detergents to remove membrane proteins as could as possible , and the cleaner inclusion bodies were abtained 其次对包涵体体外复性进行研究。用离子型与非离子型去污剂反复洗涤包涵体，尽可能去除膜蛋白。
Myeloid dendritic cells , which contribute to an immunologic synapse responsible for activation of the adaptive immune system , are abundant within muscle in inclusion body myositis and polymyositis 作用于免疫突触反应、活化适应性免疫应答的髓样树突状细胞在包涵体肌炎和多发性肌炎中大量存在。
The analysis of product solubility revealed that 5 - copy fusion protein mainly existed as inclusion body . the inclusion body was solulized in 8m urea and purified by poly - his protein purification kit 将包涵体用8m的尿素溶解后，经poly - hisproteinpurificationkit亲和纯化，获得纯度为99的蛋白。
But the mutation of this gene has not been found in those samples by pcr - sscp and this indicated that this gene might carry out its function through up - regulation or down - regulation 将表达产物破包涵体后经glutathinonesepharose4b柱亲和层析分离， sds page显示纯化的蛋白质为一分子量60kd的单一蛋白带。
Then the total 25 engineered strains were cultured and induced in the respective conditions . the results show that the target products expressed in 3 strains of them are partly soluble and active , whils 11bl21 ( 0e3 )表达的包涵体蛋白进行了变性、复性研究，部分获得有活性的d一海因酶，酶活力约为0 . 90u / mgo
This article presents a systematic pathology study of inclusion body hepatitis of avian mnong 32 million chickens of 87 chicken farms during the period of 1994 - 1995 in the central south section of hebei province 介绍了河北省中南部地区1994 - 1995年间， 87个鸡场， 3200万只鸡发生鸡包涵体肝炎的系统病理学研究。
Result bl21 with pgex - 4t - l - nk4 could correctly the targeting protein . the bacteria were cultured for 2h before being induced by iptg . the high - level production was got by being induced at 37 for 2h 结果发现:在37菌体以包涵体的形式表达，在朋菌体1 :要以可溶性状态表达，在25 “ c菌体也以包涵体形式表达。
3 r hpf4 was expressed as inclusion body in e . coli , after washing with reagent i ( 50mm tris - cl ph 8 . 0 ; 100mmnacl ; 0 . 5mm edta ; 2m carbamide ; 0 . 2 % tritonx - 100 ; 0 . 2 % doc ) and reagent ii ( 50mol / l , ph8 . 0 tris 3超卢结合溶由酶法破碎人肠杆菌后，经分析rhpf4主要以包涵体的形式存在，包涵体经洗涤液iuommtrk mphs
The changes of above show that the damage to the chicken embryo caused by the chicken parvoviruses were widly . the pathogeny of infectious stunting syndrom was prolifirated in cells nucleus , it was a dna virus 以上变化说明了分离病毒对机体损伤的广泛性。核内包涵体的出现进一步说明，鸡传染性生长障碍综合征的病原在细胞核内增殖，为dna病毒。
Coli have been performed in this study . engineering bacteria , screened for efficient expression of pheromone 3 , express the proteins in type of insoluble ( inclusion ) and soluble forms , respectively 本实验对游仆虫信息素在大肠杆菌中的表达进行了系统的比较与分析，筛选获得了有效表达信息素的工程菌株，表达产物分别为包涵体与可溶性蛋白。
