3 . shading levels effected on the growth of cultivated plantlet from adventitious buds in plastic house . lower shading level could increase the root development including root number , fresh weight and dry weight 不同遮阴度对花叶玉簪品种不定芽组培苗炼苗的营养生长有较大的影响，低遮阴度能显著增加植株的根数、根鲜重和根干重。
In particular , dsmv - dyl possesses only 76 - 83 % and 66 - 73 % sequence similarity with those documented data for cp and 3 ' - utr , while sequence identity of dsmv - dy2 and fh possess 89 - 91 % and 87 - 95 % similarity respectively with other isolates 外植体在ms 2m叭6 ba 3蔗糖0 7琼脂的培养基中形成不定芽，将不定芽转移至ms 3蔗糖0
Sonication - assisted agrobacterium - mediated transformation ( saat ) can increase the efficiency of transformation . 2s sonication can enhance the gus gene transient expression and induction of resistant buds without leaves incision 头孢毒素( cef )对不定芽的诱导有一定的抑制作用，哌拉西林钠( pip )对不定芽的诱导抑制作用稍弱，以pip400mg / l作为抑菌生长浓度。
Cotyledon and true leaves of stem mustard were cultured on ms medium supplemented with different concentrations of various cytokinins ( ba , cppu , tdz or kt ) alone or in combination with naa or iba , and the adventitious buds regeneration rate was evaluated . 以榨菜子叶、真叶为外植体进行离体培养，用ba 、 cppu 、 tdz和kt等细胞分裂素和生长素naa 、 iba组合诱导子叶、真叶再生不定芽试验。
High sucrose and agar is apt to keep the tight close structure of callus . ( 4 ) the optional inducing medium of adventitious buds is ms basal medium with 2mg / l 6 - ba , 30g / l sucrose and 9g / l agar . all the tested genotypes have high inducing rate on this medium 供试的4个基因型在此培养基上不定芽诱导效率均最高，由高到低依次为：松93 - 8为962 . 9 ；东农v10为679 . 7 ；东农419为542 . 5 ；富士光为482 . 6 ，与其它处理差异达到显著。
In general , only one bud shall be reserved on per stem , other buds shall be cut off , so that nutrition can be centralized on one bud , you can get large and bright flower . some vigorous varieties can produce 2 or 3 normal flowers on per stem , for these varieties 2 or 3 buds could be reserved 为使牡丹花大艳丽，常结合修剪进行蔬芽抹芽工作，使每枝上保留1个芽，余芽除掉，并将老枝干上发出的不定芽全部清除，以使养分集中，开花硕大。
The result of study on the factors affecting the transformation frequency showed : the leaf disc was pre - cultured for 2 days , infected for 20 minutes , co - cultivated with agrobacterium eha105 for 2 days , which could improve regeneration efficiency . the highest regeneration efficiency was 13 . 3 % in this experiment 实验结果表明：叶片预培养2d ，农杆菌浸染20min ，与农杆菌共培养2d时，筛选培养基上洋桔梗叶盘不定芽分化频率最高，达13 . 3 。
S . regeneration system of mustard hypocotyls and cotyledons of 4 days and leaves of 25 days were used as transformation materials , and ms as basic medium supplying different ba ^ naa and kt concentration , we got the optimum explant adventitious bud inducing medium ( ms + 6 - ba3 . 0mg / l + naa0 . 2 mg / l ) . among the three kinds of mustard explants . the cotyledons showed the highest adventitious bud regeneration capacity . and the regeneration capacity of leaf explantswas weaker . and the weakest of hypocotyls 芥菜高频再生体系的建立以4天左右的叶用和茎用芥菜子叶、下胚轴和25天苗龄的芥菜无菌苗的叶片为外植体，以ms为基本培养基，通过加入不同浓度和不同组合的ba 、 naa 、 kt ，筛选到了外植体不定芽分化的最适培养基( ms + ba3 . 0mg / l + naa0 . 1mg / l )及生根的最适培养基( ms + naa0 . 2mg / l ) 。
An in vitro plantlet regeneration system was established from rhizome lumps in polygonatum cyrtonema hua and mannose - binding lectin in cultured rhizome was determined by sds - page and hemagglutination assays and also analysised by rt - pcr and comparison of the nueleotide sequences and amino acid deduced with natural polygonatum cyrtonema hua lectin ii . 利用野生囊丝黄精的幼嫩根状茎作外植体，诱导形成愈伤组织，愈伤组织分化出不定芽，进而产生试管苗，试管苗经移栽试验，成活率为75 ，其外观性状与野生植株无明显差异。
