On the basis of the separation and identification of streptococcosis suis in the southern part of henan province , the certain typical bacterial strains were selected as the vaccine strains to produce polyvalent inactivity vaccine for the local prevention of the disease 摘要在对豫南地区猪链球菌病病原分群鉴定的基础上，挑选具有一定代表性的菌株作为疫苗株，研制多价灭活疫苗免疫防制猪链球菌病。
Laboratory surveillance also found an increasing number of influenza virus detection recently . the predominant strain remains to be influenza a h1n1 new caledonia . the strain matches the vaccine strains recommended in the 2005 06 government influenza vaccination program givp 化验结果显示，近期侦测到的流感病毒的数字有所增加，经病毒分离检测后发现病毒主要为甲型流感1 1新喀里多尼亚，与2005 06年政府流感疫苗注射计划所使用的疫苗合。
Because of difficulty in differentiating eiav wild strain from the vaccine strain （ dla ） in vivo ， we have to build a fast identification method between wild strain and vaccine strain of eiav s2 is an accessory protein of equine infectious anemia virus ( eiav ) , the s2 function is not fully defined 由于我国马传贫弱毒疫苗存在野毒和疫苗毒鉴别困难的问题，目前尚未得到欧美等发达国家的认证，如要进入国际市场，急需建立一种eiav强、弱毒快速鉴别诊断方法。
The result shows that the high degree of homology of e2 sequences and 426 residues amino acid sequences existed in the strain hclv , hcv - jn , hcv - yc , fc and the epitopes between the four strains are not variable obviously by analysising the variation of some main amino acid residues substitutions of e2 major antigenic domains , confirming initially immunization with hcv vaccine strain can resist challenging of field strains 结果发现， hclv与fc间核苷酸及氨基酸序列的同源性分别为98 . 3 、 99 . 2 ；两个野毒株与hclv和fc株之间的同源性也分别在98以上，与alfort株的同源性最低，分别在83 、 89左右。据此，可推测hclv 、猪瘟野毒株和c株之间的e2基因序列及氨基酸序列无明显差异。
Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f . for the construction of transfer vector pfgs11haf , aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11 . recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l . recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier Puc18ha和sk质粒同时经hind 、 kpn酶切后连接得中间质粒skha ；将质粒skha用bamhi酶切回收ha基因插入到插入载体pfgs11中的bamhi位点，通过酶切鉴定获得了pfgs11ha ；将含ndvf基因的质粒puc19f用hind 、 sal酶切经klenow酶补平插入到经sma酶切后的skifn中pe / l启动子下获得中间质粒skf ，再将质粒skf和puc18质粒先分别用ecor 、 xho酶切klenow补平，后再共同用sac酶切连接得puc18pelf ， sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位点，通过酶切鉴定获得了pe / l - f与ps - ha同向的表达载体pfgs11haf 。
Rt - pcr was used to amplify the cdna of the genome . these cdna fragments were cloned into the plasmid pucm - t . the result indicated that the seguence of the genome was obtained . the genome of aev - nh937 composed of 7055 nucleotides , potentially encodes a polyprotein of 2134 amino acids . the genome of aev - nh937 has 94 . 3 % nucleotides identity with the calnek vaccine strain of aev 把它们分段克隆在pucm - t载体上，经序列分析，获得了aev - nh937毒株的基因组全序列及推导的氨基酸序列，基因组全长为7055个核苷酸，与calnek疫苗株具有94 . 3的同源性，编码一个含2134个氨基酸的多聚蛋白。
The result further proposed that different genotypes of ndv had not directed the antigenic changes of f protein , the most important protein of ndv in the protective immune response of the fowls . protective efficacy difference among various genotype ndv vaccines of different origin was studied . except the vaccine strain lasota ( genotype ii ) , the challenge strain f48e8 in china , the ndv strains tested were isolated from nd outbreaks in chickens , gooses , and pigeons 通过不同来源不同基因型(亚型) ndv灭活苗与常规疫苗(包括lasota弱毒活疫苗和灭活疫苗)的免疫保护效力的比较，证实了所有疫苗均能激发机体产生抗不同基因型ndv的抵抗力， hl抗体变化都具有一个抗体滴度迅速上升的过程，动态特征除lasota弱毒活疫苗组有些不同外相当一致。
The research consist of four parts . the first part is multiplication , purification and electron microscope examination of the avian encephalomyelitis virus . a 1 : 5 dilution of isolate - nh937 of aev and control group of pbs were inoculated to susceptible 6 - day - old chickens embryos . respectively . after incubation for 10 days , the urinay vesicle liquid was collected . making a comparison the size of the chickens embryos between the test group and the control group , the results showed that the size of the control group is bigger than that of the test group . purified virions were examined under the electron microscope , the result revealed that there are a lot of virions and the aev - nh937 was multiplicated in embryos . the second part was seguence analysis of the genome of the aev - nh937 . nine pairs of primers were designed according to published calnek vaccine strain of aev 本研究共分四个部分：第一部分为aev的增殖，纯化和电镜观察，用1 : 5倍稀释的aev - nh937株和阴性对照pbs分别经卵黄囊接种于6dspf鸡胚，继续孵化10d后，收集尿囊液。比较接种组和健康对照组鸡胚的大小，结果显示，健康对照组鸡胚明显大于接种组。分离、提纯aev ，把纯化的病毒在电镜下观察，证明确有大量aev病毒粒子存在，说明aev在鸡胚中成功扩增；第二部分是aev - nh937基因组的序列测定工作。
The comparison of meq gene sequences of different pathotypes of marek ' s disease virus meq genes of different pathotypes of marek ' s disease virus ( mdv ) were amplified , in the whole opening reading frame ( orf ) by polymerase chain reaction ( pcr ) technique , sequenced and compared with the published sequence of ga strain ( representing vmdv ) . cvi988 / rispens and 814 , commercial vaccine strains popularly used worldwide and in china respectively ; 648a , representing very virulent plus ( vv + mdv ) and 6 field isolates , originated from guangxi commercial chickens with visceral lymphomas and vaccine breaks , representing high pathogenic ( hpmdv ) and two of them ( g2 and n ) were proved to be vvmdv , were used 本研究通过三个方面进行了研究，取得了如下主要实验结果： 1mdv不同致病型( pathotypes )毒株meq基因序列的比较研究应用pcr技术扩增了不同致病型mdvs ，即国际通用型疫苗毒cv1988 rispens株和中国特有的疫苗毒814株、特超强毒648a株( vv + mdv )以及6个广西分离到的野毒株共9个毒株的meq基因，进行核苷酸序列的测定并与标准强毒ga株( vmdv )进行核苷酸和氨基酸序列的比较。
