It also showed good cell affinity to the modified pdl - la scaffold investigated by scanning electronic microscopy ( sem ) and confocal laser scanning microscopy ( clsm ) , comparing with the unmodified pdl - la scaffold 扫描电镜和激光共聚焦显微镜的测试结果显示了软骨细胞在原浙江大学博士学位论文（ 2003 ）位自修饰的聚乳酸三维支架内部良好的粘附与生长情况。
In this paper , we introduce the function of the confocal laser scanning microscopy in the studies of protein crystal growth kinetics , such as measuring the numbers of crystal , reconstruction 3 - d image to acquire the information of crystal growth process 本文介绍了其在定量测量晶体的个数，重组三维图像以获得晶体生长的过程信息及测定晶体生长台阶动态变化等方面的应用。
In order to provide strong researching tools for the researchers in those fields to reach and exceed the world advanced level , we have developed laser confocal scanning microscopy and gene chip scanner 为了给我国在生物医学、基因工程等领域进行不懈研究的广大科学工作者提供强有力的研究工具，更好地赶超世界前沿水平，从而着手研制开发激光共聚焦扫描显微镜系统和基因芯片扫描仪。
The confocal laser scanning microscopy ( clsm ) can be a powerful tool for in situ observation and analysis of protein crystal growth kinetics , because of its high - resolution , noninvasive scanning for crystal and 3 - d reconstruction 摘要激光扫描共聚焦显微镜与普通光学显微镜相比，其分辨率高，同时具有可对样品进行非侵入性无损伤断层扫描，以及对样品形貌进行三维成建等特点，因此，可作为研究晶体生长强有利的工具。
Semiquantitative analysis of lignin content in the culm by confocal laser scanning microscopy ( clsm ) showed that lignin contents are elevated in lodging - resistant varieties and this together with the anatomical features may provide higher culm strength resulting in increased lodging - resistance 因此，在超高产小麦品种的选育上，重视外源基因的引进，改进茎秆的结构特性及提高其木质素含量，可能是一个重要的选育方向。
After treatment with bfa , immunoflurescence labeling and confocal laser scanning microscopy indicated that the cytoplasmic dynein intermediate chain - like protein in lily pollen tube was insensitive to bfa treating , but spectrin - like protein was sensitive to it under the same condition 用bfa处理百合花粉管后，通过免疫荧光标记及激光共聚焦扫描显微镜观察发现类细胞质力蛋白中间链对bfa不敏感；同样的处理发现类红膜肽对bfa敏感。
Discussed the optical imaging principle of laser confocal scanning microscopic imaging system whose important character is high plane resolution and high depth resolution . analyzed the controlling technique of scanning imaging . according to the different needs for laser confocal scanning microscopy and gene - chip scanner , two kinds of scanning methods , optics scanning and object scanning , are presented 讨论了激光共聚焦扫描显微成像系统的光学原理及其成像的重要特点，即激光共聚焦扫描显微成像系统不仅具有高的平面分辨率而且具有很高的深度分辨率；分析了系统的扫描成像控制技术，根据激光共聚焦扫描显微镜系统和基因芯片扫描仪的不同需要提出了两种扫描方式，即光学扫描方式和物体扫描方式，指出了两种扫描方式的优缺点，并对各自的成像非线性畸变问题进行了探讨，提出了解决方案。
The results of confocal laser scanning microscopy ( clsm ) and atomic force microscopy ( afm ) proved that the microspheres or nanospheres with different surface characters were obtained successfully . the clsm investigation of fitc labeled particles showed that polymeric microspheres have a core - shell structure in which the surfactants existed in the shell of particles . polymeric microspheres with different surface characters were embedded onto the surface of pla membranes via surface entrapment 激光共聚焦显微镜( clsm )和原子力显微镜( afm )观察分析结果证实可通过上述技术获得具有不同表面性质的微米级或纳米级聚合物微球，而荧光标记技术( fitc )则证实了聚合物微球具有明显的核壳型结构，表面稳定剂存在于微球的壳层。
On the base of studying imaging theory of lens , the imaging theory of laser confocal scanning microscopy was analyzed in detail in this paper , and the advancement of that the optical fiber was applied to the system was described ; on the base of completed the demonstration for whole project , the experiment scheme was designed ; the relationship between the main parameters of key devices and the resolution was deduced , and the requirements of coupling efficiency and vignetting effects to optical system was analyzed ; the design of optical system and the planar scanning controlling circuit was completed ; a new method was put forward to resolved the inherent non - liner scanning problem of the galvanometer scanner by using software liner controlling in circuit design , and the perfect planar scanning was realized ; at last the low noise , high multiple and non - distortion amplify circuit of photoelectric detector was completed 本文在透镜成像理论的基础上，系统、深入地分析了共焦扫描显微成像的机理，论述了应用单模光纤的激光共焦扫描显微成像系统的优点；进行了总体方案的论证，并设计确定了单模光纤激光共焦扫描显微成像系统的总体方案；从理论上推导分析了分辨率要求与试验系统中相关器件主要参数之间的关系，分析了系统耦合效率和渐晕现象对光学系统的设计要求；完成了方案中光学系统和二维扫描控制电路的设计，并在电路设计中采用了用软件解决检流计式光学扫描器(振镜)非线性问题的新方法，能够实现较为理想的二维模拟扫描；完成了高增益、低噪声和低失真的探测接收系统的设计和调试。
By sds - page and immuno - blotting , we found that a monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain antibody could react with cytoplasmic dynein intermediate chain - like protein at 67 kda in lily pollen . under confocal laser scanning microscopy after immunoflurescence labeling , we found that the dynein intermediate chain - like protein appeared punctated and was co - localization partly with microtubules in cytoplasm of lily pollen tube 免疫荧光标记及激光共聚焦扫描显微镜观察发现，类细胞质力蛋白中间链在百合花粉管中存在于颗粒状细胞器上；免疫荧光双标及激光共聚焦扫描显微镜观察发现，百合花粉管中类细胞质力蛋白中间链和微管存在部分共分布。
