Ip3 - ip3 receptor ( ip3r ) interaction mediates the release of ca2 + from the endoplasmic reticulum in response to many different extracellular stimulus . for higher plants , however , though it is now generally accepted that ip3 participates in signal transduction in many important cellular processes , only limited evidence is available for the presence and properties of the ip3r - like protein so far . here , using the immunological methods with an antibody raised against a mammalian inositol 1 , 4 , 5 - triphophate receptor ( type 1 ) , we found that , 1 ) the antibody across - reacted the proteins with about 200kd in microsomes from oryza sativa and about 200kd from arabidopsis thaliana respectively 本实验用sds - page电泳和免疫印迹的方法，用哺乳动物大鼠三磷酸肌醇受体的多肽做抗体对类三磷酸肌醇受体蛋白鉴定，结果表明：抗体与水稻和拟南芥微粒体蛋白分子量大约为200kd的蛋白交叉反应，同时还发现在水稻微粒体蛋白62kd和拟南芥微粒体蛋白45kd处有交叉反应的蛋白条带存在，表明在植物中有类三磷酸肌醇受体蛋白的存在；用免疫胶体金方法，发现类三磷酸肌醇受体蛋白主要分布于液泡膜和细胞质膜上。
Our data demonstrated that extracellular ca2 + was required for blue - light - induced anthocyanin accumulation and chs gene expression and verapamil sensitive plasma membrane ca2 + channel played a positive effector on bl response . heparin , an antagonist of ip3 receptor increased the anthocyanin content induced by bl but inhibited chs gene expression Ip3与cs通道受体结合抑制剂肝素处理可促进蓝光诱导的花色素苦积累，但抑制chs基因表达； ip3分解抑制剂lic13则抑制花色素着积累而促进基因表达；施加肌醇既促进蓝光诱导花色素着积累又促进基因的表达。
Additionally , protein bands were found in the range of 62 kd in microsomes from oryza sativa and 45 kd from arabidopsis thaliana - , 2 ) the ipar - like protein was mainly localized in the vacuolar membrane ( tonoplast ) and plasma membrane in the leaf and root tip of arabidopsis thaliana for confirming the biological role of the ip3r - like protein , we investigated the effects of methyl viologen ( stimulating ip3 produce ) and heparin ( a competitive inhibitor of inositol 1 , 4 , 5 - triphophate in animal ) on stomatal movement by epidermal strip bioassay . when the epidermal strips of arabidopsis thaliana were treated with methyl viologen , it stimulates the stomatal closure 拟南芥表皮条用甲基紫精处理，可以引起气孔的关闭，当处理90分钟的时候，气孔的相对开度由100降到58 ，统计学分析气孔开度的变化有显著差异；甲基紫精引起气孔关闭的作用可以被肝素部分抑制，当甲基紫精和肝素同时处理90分钟的时候，气孔的相对开度由100降到92 ，统计学分析气孔开度的变化没有显著差异，说明气孔的相对开度基本上没有变化。
