Milk and milk products - guidelines for a standardized description of competitive enzyme immunoassays - determination of aflatoxin m1 content iso 14675 : 2003 ; german version en iso 14675 : 2003 牛奶和牛奶制品.竞争酶免疫分析的标准化描述指南.黄
The quality of an enzyme immunoassays depends very much on the purity of the antigen or hapten used for conjugation , the specificity of the antibody and the choice of a suitable enzyme label 酶免疫分析技术的质量依赖于抗原的纯度、抗体的特异性、合适标记酶的选用，其灵敏度取决于标记酶的高度纯化和高转化率。
P - galactosidase of e . coli which is scarce in human blood plasma and has a variety of substrates , is one of the most frequently used enzyme labels . it is used both in heterogeneous and homogeneous enzyme immunoassay 大肠杆菌的-半乳糖苷酶，因人血浆中缺乏此酶，并具有大量易得的底物，被用于均相及非均相免疫分析，是目前最常用的标记酶之一。
A rabbit was infected with a cloned yntatl , blood was collecting from from the rabbit every 3 days after infection within 30 days , 10 clonal trypanosome populations were gotten , infecting a new rabbit by the last non - cloned trypanosome population . repeated above all , thus infected 5 rabbits sequentially . twenty different vats ( variant antigen type ) were monitored and characterized from those fifty mono - clonal populations by indirect immunofluorescence test ( ift ) and avidin biotin enzyme immunoassay ( abc - eia ) 用伊氏锥虫云南水牛单克隆株yntat1感染兔，感染后30天内，每3天从兔血中分离锥虫并单虫克隆，最后一个未单虫克隆的虫株感染另一只兔，重复以上操作，这样顺序感染5只兔子，共获得50个单克隆锥虫种群( tp ) ，经间接免疫荧光和abc酶标试验鉴定共为20个抗原性互不相同的抗原变异体( vats ) 。
Abstract : the determination of aminoglycoside antibiotics in the biological fluid has been reviewed . the methods mainly included bioassay , thin - layer chromatography , high - performance liquid chromatography ( hplc ) and a variety of immunoassay ( ia ) , for example , enzyme immunoassay , fluorescence polarization immunoassay , quenching fluorescence immunoassay , chemiluminescent immunoassay ， etc . , in which hplc and ia were used more often 文摘:综述了氨基糖苷类抗生素的体内分析方法.主要有：生物学分析法；薄层色谱法；高效液相色谱法；差示脉冲极谱及各种免疫法，如酶免疫法，荧光偏振免疫法，胶乳凝集抑制速率比浊法等.其中高效液相色谱法和免疫法应用较多
