eiav

• Eiav virions were observed by electron microscope from donkey leukocytes infected by virus derived from eiav - pok8 . 2 - his . these results demonstrate that changing the eiav s2 gene ( inserting small foreign gene fragment ) does not appear to affect replication properties in target cells in vitro
  本研究通过soe法巧妙地对s2基因引入突变，插入his标签，成功地获得了标记感染性分子克隆，证明s2基因缺失突变株在体外巨嗜细胞上培养不影响其复制力，为野毒株和疫苗毒的鉴别诊断打下基础。