In this thesis , the fist cyanophage isolated in china was investigated with regard to the molecular biological characters . techniques for the large harvest and the electronic microscopy negative staining of the cyanophage were established . the genome library was constructed 论文对我国淡水中分离得到的一株蓝藻病毒(噬藻体)的某些分子生物学特性进行了研究，建立了其大量增殖与电镜染色的技术，构建了基因组文库，并对部分序列进行了测定与和分析比较。
A new concentration system , the backflushing ultrafiltration system , was constructed with the isolated cyanophage pp in our lab associated with the ultrafiltration technique . basing on this system , the cyanophage concentration multiple could reach up to 30 times and the recovery rate of the cyanophage could exceed 90 % 浓缩是研究噬藻体的重要前期工作，我们以本实验室分离的淡水噬藻体pp为实验材料，结合超滤技术，建立了一套能够有效浓缩水样中噬藻体的方法- - -反冲超滤法。此浓缩技术可使噬藻体的回收率达90以上，浓缩倍数达30倍。
Under mixed culturing conditions , it was observed that bacterial number rapidly incre ; ised soon after the lysing of host cells . on the contrary , while the non - host cyanobacterium ( i . e . anabaena flos - aquae ) was incubated in the mix culturing system , the breeding of the bacteria could be inhibited . it may be suggested from the result above that cyanophage could bring about the changes in microorganism populations 调查结果显示， 19个采集的水样中有6个含有裂解织线藻的噬藻体，而且水样未经浓缩即能裂解宿主，说明噬藻体在淡水中分布较广泛，裂解性也较强；混合培养条件下的研究结果表明，噬藻体裂解宿主后，细菌数量快速增加，而当培养系统中有非宿主藻类存在时，细菌的增殖则受到非宿主藻的抑制，说明噬藻体可以显著改变系统中微生物的种群结构。
Again , using cyanophage pp and adopting orthogonal design , we have performed the studies on the effects of several environmental factors on the multiplication of the cyanophage . results are as follows : in the experiment of measuring the adsorption rate , temperature was found to be the primary influencing factor and displayed an obvious significance by the statistics analysis 以噬藻体pp为对象运用正交设计的实验方法，我们还研究了几种环境因子对噬藻体增殖的影响，结果如下：在测定吸附率的实验中，我们发现温度是主要的影响因素，并且在统计学上有显著意义。
As temperature rose , the saturated adsorption rate tended to increase , and the time to reach saturation would become longer . comparatively , the sunlight radiation showed a minor influence . with the intensity of sunlight radiation strengthened , the adsorption speed of the cyanophage to the host gradually quickened , and reached the maximum of 62 . 4 % at 28cand 2000 lux 随着温度的提高，饱和吸附率呈不断上升的趋势，达到饱和吸附率的时间也不断延长；与温度相比，光照是次要的影响因素，随着光照强度的增加，噬藻体对宿主藻的吸附速度不断提高，饱和吸附率不断上升，达到饱和吸附的时间也不断延长。
Preliminary studies on the ecological characters of freshwater cyanophage were carried out under both field condition and culturing system . the field investigation result showed that 6 in 19 water samples contained cyanophages lysing plectonema and the lysis phenomenon were readily observed without concentrating the water 对淡水噬藻体的生态学进行了初步研究，包括淡水中噬藻体分布及感染力的调查与人工混合培养条件下噬藻体裂解宿主引起微生物和藻类种群变化的研究。
