Conditioned mediums of different rat cerebral microvascular endothelial cells against damage of ischemia and ischemia reperfusion neurons 不同脑微血管内皮细胞条件培养液抗缺血及再灌神经元损伤的比较
Therefor , granulosa cell conditioned medium may be benefit for oocytes maturation , and it has not yet been fully studied 在成熟培养体系中引入颗粒细胞的生长液能否促进卵母细胞的完全成熟，尚未见研究报道。
Neurons and neurogliocytes of spinal cord were cultured with conditioned medium in various concentrations of human embryonic neural stem cells 采用免疫组织化学方法对分离培养的神经干细胞及培养的脊髓神经细胞进行染色鉴定。
Influence of different endothelial cells conditioned medium on the function of mitochondria of cortical neurons and the protective effect of tongluo jiunao injection 不同内皮细胞条件培养液对皮层神经元线粒体功能的影响及通络救脑注射液的保护作用
Abstract objective to explore the effect of conditioned medium of various concentrations of human embryonic neural stem cells on the growth of cells in the cultured spinal cord tissues in vitro 摘要目的探讨不同浓度神经干细胞条件培养液对体外培养脊髓组织中不同细胞成分生长的影响。
Conclusion the effect of conditioned medium of human embryonic neural stem cells can improve the growth of the spinal cord neurons and neurogliocytes cultured in vitro according to its concentration 结论人胚胎神经干细胞条件培养液对体外培养的脊髓神经元或胶质细胞促生长作用的差异与其浓度有关。
To study the effects of granulose cells conditioned medium , eof and igf - 1 on in vitro matured kunming mouse oocytes . 4 . to utilize cgs and microtubules as the markers of mature cytoplasm to assess the efficiency of different cultured systems 研究卯巢颗粒细胞主长液、表皮生长因子（ epidermalgrot4thfactor ， eg ）和胰岛素样主长因于1 （ insulinakegtotvthfactor ， igfl ）对卵母细胞体外成熟和发育潜能的影响； 4
In order to simplify the process of preparing conditioned medium , we also immortalized rat myocardial cells . these methods broadened the conditions for the establishment of es cell lines and open up a new point to study the stability of cultured es cells 同时，还对大鼠心肌细胞进行了永生化的尝试，共得到了4个永生化克隆，这将进一步简化es细胞建系和培养工作，为进一步研究es细胞在体外培养过程中的稳定性开创了新的起点。
The methods of evans and martin were changed slightly and used to isolate the mouse es cell in my experiment . in brief , the intact blastocysts were plated on sto feeder layer treated with mitomycin , and were cultured in the media supplymented with brl condition medium 联合evans和martin的方法，稍加改良来分离小鼠胚胎干细胞，把昆明白小鼠完整的囊胚直接种植在经丝裂霉素灭活的sto饲养层细胞上，在含有brl条件培养基的es细胞培养液中培养。
The second medium included egf ing / ml and igf - 1 long / ml in addition to the control . likewise , the last medium contained 50 % granulosa cells conditioned medium . oocytes were cultured for 16 or 18 hours , and only the oocytes underwent first polar body extrusion were collected for future study Inmegflug ml十igf 110ng ml ）和颗粒细胞生长液组乃0颗粒细胞生长液） ，对昆明白小鼠生发泡期（ ge讪nalvesicle ， gv ）卵母细胞进行体外成熟培养； 5
