Methods subacute senile mouse model was established by injection of d - galactose . the splenic cells were isolated from mice and cultured in vitro following cona stimulation . expression of cd 137 on splenic t cells in the model and normal control groups were compared with each other by flow cytometry method at various cultural times after cona stimulation 方法取5周龄balb c雄性小鼠，颈背部皮下注射d -半乳糖，分别于造模型后一到五个月取脾脏淋巴细胞，同时取自然衰老小鼠脾脏淋巴细胞，在培养的第0 、 24 、 48 、 72 、 96 、 120 、 144h收集细胞，用流式细胞仪分析脾脏t细胞cd137的表达阳性率。
The hsp70 mrna level in the hepatopancreas of agonal shrimps infected with wssv by feeding was lower remarkably than that of normal shrimps , while no rule of hsp70 transcription was discovered in muscles of shrimps infected with vibrio anguillavium and wssv for different hours by injection 但对经注射wssv病毒及焦传珍编码中国对虾和凡纳对虾一种卜isp70的cona研究博士学位论文鳗弧菌后不同时间的对虾肌肉组织进行hsl770mrna水平检测，未发现两种病原感染不同时间后hsl刃0表达的明显规律。
Time courses of administration in these three groups were different ( group - 2 : day - ( - 3 ) day - 11 ; group - 3 : day - 0 day - 14 ; group - 4 : day - ( - 3 ) day - 0 ) . group - 5 were treated with cyclosporine ( csa ) ( 10mg / kg / d ) and group - 6 were treated with rad ( 3mg / kg / d ) by daily oral gavage from day - ( - 3 ) to day - 11 . in group - 2 , the peripheral lymphocytes number and t cell population were monitored 体外培养小鼠脾细胞，加入fty720 （ 10 m ） ，检测dna改变及细胞膜表面annexinv结合率，还在cona刺激脾细胞增殖的培养体系中观察了fty720 （ 10 m ）对t细胞表面cd25 ， cd69表达的影响。
