According to the research of physiological and biochemical indicators or index , components of soluble proteins , substrate protein of phosphorylation and the activity of protein kinase in low - temperature stress in the leaves of brassica oleracea l . , we tried to find the law of the physiological and biochemical response of brassica oleracea l . leaf to low temperature . at the same time , discussion on the signal transduction can also provide further evidences for revealing the mechanism of low - temperature stress . the results are showed as follows : malondialdehyde ( mda ) , superoxide dismutase ( sod ) , ascorbate peroxidase ( asp ) and peroxidase ( pod ) activities were changed greatly after 0 ~ 30min ' s treating with low temperature 本文以甘蓝叶片为材料，通过对低温5胁迫下甘蓝生理生化指标、可溶性蛋白组分以及磷酸化底物蛋白、蛋白激酶活性的研究，以期找出甘蓝叶片对低温胁迫的生理生化响应规律，为甘蓝露地越冬栽培防范寒害提供理论指导，同时对低温胁迫下甘蓝逆境信号传导进行了探讨，从而为彻底弄清低温胁迫机理提供进一步的证据，研究的主要结果如下：丙二醛含量( mda ) 、超氧化物歧化酶( sod ) 、抗坏血酸过氧化物酶( asp )和过氧化物酶( pod )活性在低温处理0 30min发生显著变化，低温处理3min后，甘蓝叶片内mda含量基本没有变化，处理5min时出现第一个峰值，达到对照的104 . 10 ， 10min出现低谷，仅为对照的86 . 27 ，随后再次上升， 30min时超过第一峰值，为对照的113 . 93 。
The method of ctab was specifically improved for the extraction of genomic dna and total rnas from brassica oleracea l . and brassica napus l . 2 . arclboler gene was amplified by pcr with template genomic dna and cdna from roots , leaves , petals , stigmas and ovaries during bud stage in brassica oleracea l . ( 200110197 ) and brassica napus l . ( y578 - 2 ) 以甘蓝( 200110197 )和油菜( y578 - 2 )自交不亲和系的植株为材料，提取幼苗中的gdna及花蕾期的柱头、子房、花瓣、叶片、根的mrna ，并对mrna进行反转录合成cdna ，然后分别以gdna与cdna为模板扩增arc1蛋白编码序列。
However , there are still some problems that cannot be avoided , such as construction of full - time supervising system for qualitative safety are lagged ; it lack the service center for science , technology and innovation , so it is too slow to generalize the use of a standard technology ; the contradiction of planting structure are relatively obvious , the comprehensive motivation of industry are too weak ; the construction of information service system are not so perfect ; etc . in order to solve the problem that limited the progress of industrial development of brassica oleracea l . var 但也存在一些无法回避的问题，如全程质量安全监控体系建设滞后；缺少科技创新服务中心，标准化技术推广应用缓慢；种植结构性矛盾比较突出，产业综合带动力弱；信息服务体系建设不够完善，等等。论文针对台州西兰花产业发展过程中存在的问题，进一步分析了促进西兰花产业发展的具体对策和措施： ( 1 )利用wto规则，依靠科技创新，全面提高西兰花出口的综合竞争力。
In thl1 gene , there are some famililar enzyme digest sites including bamh i , hind , sac i and sal i . the sequence of thl1l from brassica oleracea l . shows 99 % identity to the sequence of thll from brassica napus l . there are some phosphorylation sites and an active site of thoiredoxin h members consisting of cppc in thll protein 甘蓝和油菜thl1基因序列有5个碱基的差异，而氨基酸仅有2个氨基酸的变异，同源性达99 。在thl1蛋白的氨基酸序列中发现了一个硫氧还蛋白家族的活性位点( cppc )和多个磷酸化位点。
