The full orp encodes a 487 - amino acid protein with a calculated molecule weight of 53 . 484 kda and an isoelectric point of 6 . 75 . at the primary sequence level , it shared high homology with clr , so was named clr - like serine protease , clsp . the clsp sequence has been submitted to genbank / embl ( genbank accession number af178985 ) 该蛋白在核酸和氨基酸水平上与人补体组分cl :表现高度同源，故将其命名为补体cl :样丝氨酸蛋白酶(旦lr一like旦erineprotease ， clsp ) ，该基因已经在ge心ank登录(登录号为af17s985 ) 。
( accession numbers : ay184425 - ay184440 ) . combined with other several full 12s rrna sequences of chinese water deer , montijac , water deer , cow , horse , goat and tragulus retrieved from genbank database , the whole set of sequences was utilized for downstream analysis based on theories and models in molecular phylogeny , whereas the secondary structure of 12s rrna was predicted and analyzed based on present small subunit of ribosome model 将上述序列与从genbank检索到的獐、麂、水鹿、牛、马、羊、鼷鹿的12srrna基因全序列一起，通过应用分子系统学理论与模型分析序列数据，以及对12srrna二级结构的分析，得出了以下主要结论： ( 1 )用最小进化方法( me )和邻接法( nj )对12srrna基因的全序列构建分子系统树的拓扑结构基本相同。
These two subspecies are away from more than about 1 200 kilometers each other . the genomic dna of microtus fortis calamorum and microtus fortis fortis were extracted and amplified by pcr according to the specific genomic dna sequence of microtus fortis reported previously ( accession number in genbank : af277394 ) 根据东方田鼠基因组dna序列片段( genbank登录号： af277394 ) ，用pcr技术扩增东方田鼠长江亚种( microtusfortiscalamorum )和宁夏指名亚种( microtusfortisfortis )基因组dna ，得到一670bp左右的特异扩增片段。
Part 1 : identification of a novel gene , tsarg2 , and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell , and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis . to rapidly attain human novel gene full - length cdna sequence , the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe , which was significantly changed in cryptorchidism and represents a novel gene . furthermore , a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line 本研究分为三个部分，其主要实验方法及实验结果如下：第一章tsarg2基因的克隆与序列分析从已获得的在隐睾和正常睾丸对照中表达量有明显差异的est片段( be644542 )入手，设计了基因特异性引物和载体特异性引物进行巢式pcr扩增，结合人类基因组草图搜索法，从睾丸cdna文库中快速分离出人类睾丸凋亡相关基因的5末端而获得全长cdna ， genbank登录号为ay040204 ，同时应用生物信息学的方法克隆了该基因在小鼠中的同源基因， genbank登录号为af395083 。
