随机引物

• 2 . twenty primers , which could amplify polymorphism in 15 chinese tea clonal germplasms , were selected from 100 arbitrary 10 - mer primers . a total of 1050 loci with an average 52 . 5 loci per primer and 70 loci per germplasm were amplified
  2 、从100个随机引物中筛选出20个用于对我国15份茶树(种内)资源遗传多样性的rapd扩增，共得到1050个位点，平均52 . 5个引物， 70个资源。
• Finally , the variant line tc243 - 2 , was analyzed by rapd - pcr amplification with 100 random primers . the results suggested that phenotypic variation came from exogenous dna transformation rather than exogenous pollen contamination . a mutation corre
  用100条随机引物对tc243一株系的rapd一pcr扩增结果表明该株系的变异不是来自外源花粉的污染，同时发现了特异条带5270 _ 1600对应的变异在tz一ts代能连续稳定遗传。
• In rapd analysis with 11 arbitrary 10 - bp primers , the 11 isolates were clustered into 7 groups based on their similarities . the similarity coefficients are 0 . 23 - 0 . 93 among groups . results of aflp analysis , with 4 selective primer pairs , are consistent with that of rapd
  用11个随机引物对这11个旗草内生真菌分离物基因组dna进行rapd分析，根据相似系数进行聚类，这些分离物被聚类为7组，组间的相似系数为0 . 23 0 . 93 。
• The rapd - pcr conditions were optimized by example experiments of two dna templates from different ecological regions , and 18 of 40 arbitrary decamer nucleotide primers were also screened with good amplication and repetition . the 17 sets of dna fingerprinting patterns were sharply made for 20 basidiocarps tested
  从40个随机引物中筛选到18个扩增效果好，重复性稳定的引物以介导不同来源的20个松茸子实体dna的扩增，得到了它们清晰可辨的dna指纹。
• The main results are shown as below : 1 . 80 individuals from 4 populations ( 20 from each population ) of argopecten irradians were analyzed by using 20 random rapd primers . 132 rapd bands ranging from 230 to 2800bp were recorded with an average of 6 . 6 bands gained by per primer
  采用20条随机引物对4个群体80个个体（每个群体20个个体）进行了rapd群体遗传多样性分析，共扩增出132个位点，片段大小在230 - 2800bp之间，平均每条引物的扩增带数是6 . 6条。
• Among 54 arbitrary primers tested , only 9 primers could generate clear and reproducible fragments in 32 species belonging to 12 genera of the family gryllidae ; 9 primers in 15 species belonging to 7 genera of the family trigonidiidae ; 12 primers in 3 species belonging to the genus oecanthus of the family oecanthidae ; 12 primers in 4 species belonging to 3 genera of the family eneopteridae ; 13 primers in 1 species belonging to the genus homoeogryllus of the family phalangopsidae ; 13 primers in 1 species belonging to the genus sclerogryllus of the family sclerogryllidae ; and 13 primers in 1 species belonging to the genus eulandrevus of the family gryllomorphidae
  本研究经用54种随机引物对57种蟋蟀的rapd扩增分析得出以下结论： 1 ）采用rapd技术，首次较系统地对中国蟋蟀总科7科26属共57种蟋蟀进行了系统学研究。在所试用的54种随机引物中筛选出可扩增出清晰可辨且稳定的rapd图谱的引物分别为：蟋蟀科9种、岭蟋科9种、树蟋科12种、结蟋科12种、蛛蟋科13种、铁蟋科13种、貌蟋科13种。
• 92 distinct bands including 87 polymorphic bands and 24 specific bands were amplified from the genome dna of gastrodia elata bl . by using these selected primers belong to groups of opb , opi , opn , op w . the rate of polymorphic bands and the rate of specific bands were 93 . 5 % and 25 . 0 % respectively . seven often samples excluding tl , t3 and t9 were identified by the 24 specific bands
  随机引物opb - 5扩增出的特异性条带最多，有4条，特异性条带率达57 . 2 ，可以鉴定出m和t8两个样品；随机引物opn - 10扩增出的9条带中，有3条特异性的带，可区分m ， t8 ， t2三个样品。
• By sun , bo ( major : marine biology ) directed by professor zhang , guofan and dr . liu , xiao population genetics of four argopecten irradians cultured populations was analyzed by using random amplification polymorphism dna ( rapd ) techniques . and partial dna sequences of the internal transcribed spacers ( its ) from four representative samples ( one sample represent for each population ) were amplified by pcr and sequenced in order to stress the phylogenetic relationships of the four populations . besides , by rapd technique , two argopecten irradians families were studied to examine whether the hybridization experiments were successful and which family was more suitable to be materials in future genetic linkage map construction
  本文以海湾扇贝4个养殖群体为研究对象，采用随机引物多态性dna （ rapd ）技术进行了群体遗传学研究，并尝试使用4个群体中代表个体的内转录间隔区（ its ）序列比对分析结果作为参考；另外，本文还运用rapd技术对人工异体交配的2个海湾扇贝家系的杂交成功率进行了分析，均获得成功证实，并评估了两个家系构建遗传连锁图谱的潜力。
• Lastly by using the technique of dot blot hybridization , the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer . the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe . by comparing the diagnostic ways of nucleotide probe and fc , the technique of nucleotide probe were proved to have high sensitivity and speci fi city
  最后，用地高辛随机引物法标记成momp基因核酸探针，斑点杂交检测衣原体基因组dna ，灵敏度可达10pg ，且不能检出其它病原体的核酸。将核酸探针法与补体结合反应法对衣原体感染的诊断进行比较，初步证明该探针具有较高的敏感性与较强的特异性。