After adding culture mediem of stably transfected jurkat cells to hepatocarcinoma cells , the binding specificity of the scfvs with hbsag was further confirmed by observation by fluorescence microscope , indirect immunofluorescence and flow cytometer analysis . prokaryotic expression plasmids ptat - ha - scfvs were successfully constructed 建系细胞培养上清与肝癌细胞作用后，经荧光显微镜观察、间接免疫荧光及流式细胞仪检测进一步确定表达的scfv融合蛋白具有与hbsag特异性结合的活性。
This understand of stored nitrogen compounds restricted seriously the progress in the investigation of vegetative storage proteins . in the dissertation , we studied more extensively the cytology , biochemical properties and biological roles of vegetative storage proteins in swietenia macrophylla and in hevea brasiliensis by light - and electron microscopy , sds - page , page , immuno - blotting , indirect immunohistochemical localization and colloidal gold labelling and cdna clone techniques 采用光镜和电镜技术、 page 、 sds - page和免疫印迹技术、电泳凝胶过碘酸? schiff试剂染色、间接免疫荧光和电镜免疫细胞化学定位技术以及cdna克隆技术，较深入地研究了大叶桃花心木和巴西橡胶树的营养贮藏蛋白质的细胞学、生物化学性质和生物学功能。
In this paper , wheat cultivars lovrin 10 ( resistant ) and 5389 ( susceptible ) were selected as materials in this system . the mesophyll protoplasts of wheats ( mpw ) were isolated using cellulase and pectolase digestion . by the indirect immune fluorescent labeling , microtubules ( mts ) pattern in mpw were showed clearly under the confocal laser scanning microscope ( clsm ) 本试验以抗(洛夫林10 ) 、感( 5389 )不同的两小麦( triticumaestivuml . )品种为材料，采用酶解法制备小麦叶肉原生质体，利用间接免疫荧光方法结合激光共聚焦扫描显微技术对小麦叶肉细胞原生质体的微管骨架进行了清晰的标记，并探索了微管骨架标记的影响因素。
The sucking mouse brain were inoculated with mdj - 01 strain to make electron microscopic examination , results showed that the virus was a spheral particle with membran which had a diameter of about 40 nm . by indirect fluorescent antibody test mdj - 01 strain was identified with tbev . a part of region encoding e protein was expanded by rt - pcr and sequenced . the nucleotide sequences of two strain viruses were compared with sequences in genbankjsequence homology analyses revealed mdj - 01 strain and senzhang strain had the highest homology with tbev oshima5 - 10 , respectively , which were 95 % , 94 % . mdj - 01 strain was identified with tbev again 应用间接免疫荧光试验进行血清学鉴定，结果表明mdj - 01株为tbev 。通过rt - pcr技术扩增部分e蛋白序列并测序，在genbank上进行同源性比较，发现mdj - 01株和森张株与tbevoshima5 - 10株的同源性最高，分别为94 、 95 ，从分子生物学水平上进一步证明mdj - 01株病毒为tbev 。在鉴定的基础上，本实验对两株病毒进行了核苷酸全序列测定。
A rabbit was infected with a cloned yntatl , blood was collecting from from the rabbit every 3 days after infection within 30 days , 10 clonal trypanosome populations were gotten , infecting a new rabbit by the last non - cloned trypanosome population . repeated above all , thus infected 5 rabbits sequentially . twenty different vats ( variant antigen type ) were monitored and characterized from those fifty mono - clonal populations by indirect immunofluorescence test ( ift ) and avidin biotin enzyme immunoassay ( abc - eia ) 用伊氏锥虫云南水牛单克隆株yntat1感染兔，感染后30天内，每3天从兔血中分离锥虫并单虫克隆，最后一个未单虫克隆的虫株感染另一只兔，重复以上操作，这样顺序感染5只兔子，共获得50个单克隆锥虫种群( tp ) ，经间接免疫荧光和abc酶标试验鉴定共为20个抗原性互不相同的抗原变异体( vats ) 。
The two isolates were positive reaction hi polymerase chain reactions with two pair of primers specific for alv - j and gave antigenically strong reaction hi the indirect fluorescence antibody assay ( ifa ) with alv - j specific monoclonal antibody je9 . negatively - stained electron microscopic and immune - electron microscopic observation demonstrated that viral particles of the inner mongolia and shandong isolate of alv - j , respectively designated imc10200 and sdc2000 strain of alv - j , showed characteristic morphology of alv 利用pcr和间接免疫荧光反应进行鉴定，两株j亚群禽白血病病毒可以被两对alv - j特异性引物扩增(特异条带约2 . 2kb和545bp ) ，且在特异性单克隆抗体je9的间接免疫荧光检测中呈现强阳性荧光反应。
Using indirect immuno - fluorescein staining method , the localization of sp - 10 , tmdc - iii and aeg on human , mouse , rabbit , pig , bull sperm was observed under confocomicroscopy . it was found that antigens sp - 10 and aeg express on acrosome of all kinds sperms , supporting their roles in sperm - egg fusion . it was firstly described that tmdc - iii express mostly on the equator , neck and some part of tail of human sperm , on the acrosome of mouse and bull sperms , on the acrosome and neck of pig sperm , on the head , neck , and middle piece , principal piece of tail of rabbit sperm 利用间接免疫荧光法对aeg 、 sp - 10 、 tmdc -蛋白在人、小鼠、兔子、猪、牛精子上进行了精确定位， aeg 、 sp - 10在顶体部均有表达，确证了它们与精卵融合有关：首次对tmdc -蛋白在人、小鼠、兔子、猪、牛精子进行了精确定位，它主要存在于人精子尾部主段，小鼠和牛精子顶体，猪精子顶体和颈部，兔精子头部、颈部和尾部的主段及尾段。
