5 and was virtually inactive above ph 6 . 0 . when the enzyme was incubated at various ph values at 37 ? c for 30 min in the absence of substrate and the residual activity was measured , the phytase was found to be stable at the ph range of 2 to 8 该酶反应的最适ph值为5 . 5 ，在phz一6之间都有较高的酶活性， ph大于6时植酸酶的活性迅速下降，在phz一8之间的稳定性很好，活性> 70 % 。
Different kinds of biochemical reaction approaches for the goal of detection and kinetics studies on microfluidic chips are reviewed , including enzymatic assay , immunoassay , enzyme - linked immunosorbent assay ( elisa ) , polymerase chain reaction ( pcr ) and nitric oxide release reaction 摘要综述了近几年来在微流控芯片上以检测或动力学研究为目的而进行的各种生物化学反应技术，包括酶反应、免疫反应、酶联免疫反应、 pcr反应和含氮氧化物释放反应。
Encapsulate some complex reaction types as objects , including elementary mechanisms , enzymatic mechanisms and gene expression mechanisms , simplify the description of complex biochemistry reactions ; at the same time encapsulate typical biological processes , such as transcription and translation , so it can avoid a lot of repeated workload 把复杂生物化学反应封装成对象,包括基本的化学反应类型,酶反应机制和基因表达机制等,简化了复杂生化反应的描述;同时对经常使用的典型生物过程,例如转录、翻译过程等,封装成对象,避免了大量重复性的工作,方便用户的使用。
Its km for sacilin at50 andph5 . owas3 . 73mg / ml . with the analysis of kinetics , the p - glucosidase showed synergistic action to the endoglucanase and the endoglucanase inhibited the p - glucosidase . the mechanism for their interaction was explained that the endoglucanase was combined with its products and the inhibition of these products to endoglucanase was removed by its hydrolysis by p - glucosidase 经两组分共同作用的酶反应动力学分析后发现，两组分这种相互作用机制的核心是：内切酶和?葡萄糖苷酶与内切酶的水解产物，也是其底物类似物? ?纤维二糖和纤维寡糖的结合以及-葡萄糖苷酶对该产物的水解。
