The results showed that : significant increase in dna migration and comet frequency in the spleen , thymus , bone marrow and peripheral lymphocytes were induced after intraperitoneal treatment of mtx at a dose of 1 . 25 ~ 5mg / kg . no obvious increase in dna single strand breaks was observed in the liver , kidney , testicle and stomach of the mice with the same treatment . the migration of nuclear dna and comet frequency of spleen , thymus , bone marrow and peripheral lymphocytes in the dose - response study showed a dose - dependent increase 实验结果：腹腔注射5mg kgmtx后3h可诱发小鼠体内脾细胞、骨髓细胞、胸腺细胞和外周血淋巴细胞的dna单链断裂且随时间延长损伤程度增大，于12h达到高峰，但未观察到对肝、肾、胃和睾丸细胞的山窗民科大学硕士学位论文影响;核dna损伤程度随着用药剂量的增大而增大。
