The subunits of dpl were dpl - a 39 . 6kd and dpl - 3 36 . 8kd , respectively . it may consist two a subunits and two 3 subunits without disulphide bridges . dpl showed no specific agglutination with any type of human erythrocytes 纯化的dpl在page和等电聚焦中显示一条带，在非还原和还原sds - page中呈现两条带，分子量分别为- 39 . 6kd和- 36 . 8kd ，证明dpl由两个亚基和两个亚基组成，无二硫键。
A page gel , stained by the petiodic acid - schiff ' s method to reveal glycoproteins , further displayed that the bindng - protein was a glycoprotein belonging to lectin , which contained 17 . 4 % ( w / w ) neutral carbohydrate content of the glycoprotein detected by the phenol / h2so4 method . peptide mapping was comparable to the reported protein in protein bank . the database homology search ( ncbi blast ) indicated that the binding - protein shared 70 - 80 % homogeneity to l - aspartate oxidase , aspartyl / glutamyl - trna ( asn / gln ) amidotransferase subunit b , glutamyl - trna reductase , histidyl - trna synthetase 连续梯度聚丙烯耽胺凝胶电泳、 sds一聚丙烯酞胺凝胶电泳和等电聚焦的结果表明该蛋白分子量为1 “ . skda ，由二个相同的亚基组成，亚基分子量为” . ikda ，等电点为8 . 25 .糖蛋白染色结合考马斯亮蓝染色的结果证实此结合蛋白是个糖蛋白，其中糖含量为17 . 44 % ，蛋白含量为82 . 56 % .凝集反应确定该糖蛋白也是一个凝集素
Activity of alcohol dehydrogenase and phenylalanine ammonia - lyase varied during the blooming of jasmine flower . the non - specific esterase isozymes were isolated by different methods . two enzyme stripes were gained with page ( polyacrylamide gel electrophoresis ) method , while eight stripes were obtained with ief ( isoelectrofocusing ) method 等电聚焦法分离茉莉花酯酶的效果较好，用聚丙烯酰胺常规分离只得到2条酶带，而用等电聚焦法分离茉莉花酯酶同工酶可获得8条比较清晰的酶带。
