If a mouse did n ' t reach termination within 2 min , the time was assigned a score of 120s . 3 . the effect of xas on ache in hippocampus and ngf in sub - maxillary gland mice were randomly grouped into blank control , xas groups 文冠果皂贰对小鼠海马内胆碱酯酶（ ache ）和颌下腺内神经生长因子（ ngf ）活性的影响将昆明种小鼠随机分为空白对照组、文冠果皂贰不同剂量组。
Much difference of the t cell subpopulations was observed among individuals after immunization . the percentages of cd4 + , cds + or cd4 + / cd8 + t cells of the animals immunized with dna vaccines increased at different extents compared to the pires1neo - immunized or mock - immunized animals 与空白对照组和空质粒p旧esineo免疫组比较，各重组质粒免疫组动物的cd4 、 c08及cd4 ic08 t细胞有不同程度的升高。
The sds - page electrophoretic patterns of the hemolymph protein showed that the proteins types changed slightly , and only one new protein with 43kda molecular weitht appeared . however the concentrations of some proteins increased or declined obviously 用broadfold法测定绿僵菌孢子诱导后的蜚蠊血淋巴蛋白质的浓度，发现注射后4h内蜚蠊血淋巴蛋白质浓度显著下降，但4h后开始回升， 24h时就超出空白对照约60 。
Collect three groups culture supernatant for elisa experiment , rt - pcr and elisa results indicated that both transcription and translation of two cytokine genes can be enhanced obviously . rt - pcr results showed that the transcription level of il - 1 and tnf - a genes of oligochitosan contrast group were as 2 Rt pcr结果表明两种细胞因子基因转录水平明显提高，加糖对照组il一互p和tnf a基因转录水平分别为空白对照组的2
5 . prepare the oligonucleotide microarray add 16 prepared probes into 384 - pole plate , meantime , add p2 , low homologic probes and probe buffer in the same concentration as positive control , blank control and negative control respectively . spot microarray as designed by the spotting machine 5 .寡核昔酸微阵列的制备以同等浓度的反义链引物咫作为阳性对照，以探针缓冲液作为空白对照，以无关探针作为阴性对照，与制备好的16条寡核昔酸探针，同时加人384孔板。
This study evaluated the biomechanical properties of the femur - acl - tibia complex following acl reconstruction with the medial one - third pt autograft . experiment investigation random contrast balance , forty - two new zealand white rabbits was divided into three groups . bpb autograft contains blood vessels group ( i ) , bpb autograft which have no blood vessels group ( ii ) , blank contrl group ( illgroup ) . thirty - six new zealand white rabbits underwent acl reconstrution using a medial one - third pt autograft 实验研究在随机、对照、均衡的原则下进行，将42只新西兰成年大白兔随机分为带血管蒂的bpb移植物组(组) 、不带血管蒂的bpb移植物组(组)和空白对照组(组) ，分别予相应处理造模，然后在术后8周、 12周、 16周取股骨? ? acl ( bpb移植物) ?胫骨标本进行生物力学测试。
Here we managed to make cultured mice peritoneal macrophages be directly influenced by oligochitosan , and be stimulated by ifn - r before oligochitosan added , then measured the changes of gene transcription and translation level of both il - 1 and imf - a , respectively by methods of relatively quantitive rt - pcr and elisa . first , rt - pcr results showed that 18 hours was the most effective time and 40ug / ml was the most effective concentration of oligochitosan , then by the same method , confirm that 4hours is the most effective time and loou / ml is the most effective concentration of ifn - r stimulating . because ifn - r can enhance il - 1 and tnf - a gene expression of macrophages alone , so add ifn - r to microphages alone for 22 hours , then examined by rt - pcr , the results showed that il - 1 and tnf - a gene expression have no remarkable difference compared with the blank contrast group 此外，由于ifn y单独作用也可促进两种细胞因子基因表达，故在巨噬细胞中加入ifn y单独作用22h ，再经阿一pcr检测，发现加ifn y的实验组细胞的几一lp和tnf a基因转录水平与空白对照组相比较无显著性差异，可见，壳寡糖和ifn v对巨噬细胞il lp和tnf一口基因转录水平的影响在作用时间上无一致性，在壳寡糖作用最适时间时，仅受ifn y刺激的巨噬细胞il lp和tnf q基因转录己下降至刺激前水平，因此可以认为， ifn y的加入仅起到对巨噬细胞预刺激使之处于敏感状态的作用，有利于增强壳寡糖对巨噬细胞的作用。
This means that joining of ifn - yonly to stimulate and make the macrophages in sensitive state , help to strengthen the enhancing effect of oligochitosan on il - 1 and tnf - a gene expression of macrophages . after confirming the most effective time and the most effective concentration of oligochitosan and ifn - y , select three groups of cells , experiment group join loou / ml ifn - r , after 4 hours , add 40ug / ml oligochitosan ; oligochitosan contrast group add oligochitosan only ; blank contrast groupadd neither ifn - y nor oligochitosan , then the three goups culture 18 hours together 在确定了壳寡糖和ifn y的最适作用时间及最适作用浓度之后，选取三组细胞状态良好，数目比较一致的巨噬细胞，实验组中加入100u mlifn v预刺激4h后，再加入40ug ml壳寡糖，加糖对照组则只加40ng砌l壳寡糖，空白对照组两者都不加，继续培养18h 。
The experimental group were muscularyly injected with il - 2 at intervals . the results were as follows : ( 1 ) the results of cellular imnune assay ( mtt ) the sixth week after the vaccination , peripheral blood lymphocytes isolated were tested by iftt . the result showed that the nonspecific od value and the specific ( x ) value of guinea pigs of il - 2 group was significantly higher than those of bcg and sham injection group 结果发现： ( 1 )免疫第二周后， bcg + il - 2组特异性和非特异性淋巴细胞转化实验所测ltt - od值与bcg组和空白组无明显差异( p 0 . 05 ) ，第四周时检测数值开始出现升高，第六周时ltt检测结果显示il - 2 + bcg组的特异性和非特异性的ltt - od值极显著高于bcg组和空白对照组( p 0 . 01 ) 。
The femur - ligament - tibal complexes were evaluated at 8 , 12 , 16 veeks postoperatively for tensile load to falure properties . the result indicate the biomechanics properties of the group i and the group ii are much more lower than the group iii . the biomechanics properties of the group i is higher than the group ii 最后结果显示带血管蒂的bpb移植物组和不带血管蒂的bpb移植物组在术后的三个时间点生物力学性能均远远低于空白对照组，而带血管蒂的bpb移植物组的生物力学性能优于不带血管蒂的bpb移植物组，三者之间均有显著性差别( p均0 . 01 ) 。
