Spla 2 can significantly inhibits egf - induced egf receptorautophosphorylation , which also resulted in the inhibitionon egf - induced phospholipase d activation and tyrosine phosphorylation of phospholipase c - g 1 . under the same condition , spla 2 can induce intracellular ceramidegeneration . moreover , exogenous ceramide analogs can also significantly inhibit such biologicalevent induced by egf , suggesting that ceramide may play an important role in spla 2 - induced cell death and the signaling events 在相同的条件下，分泌型磷脂酶a亦能诱导细胞内神经酰胺生2成的增加；外源性的神经酰胺类似物也能有效的抑制表皮生长因子诱导的这些生物事件，提示神经酰胺在分泌型磷脂酶a所介导的细胞死亡和信号传导事件2中起着重要的作用。
Both spla 2 and ceramidehave been shown to inhibit bradykinin - , and pma - induced phospholipase d activation . these results indicated the existence of a subtle intertwined regulatory interaction between intracellular phosphoglycerol lipids and sphingolipids signaling pathways . in addition , we also found that spla can 2 down regulate theepidermal growth factor ( egf ) receptor activation 此外，我们还发现分泌型磷脂酶a能下调表皮生长因子受体的活化，它能明显的抑制表皮生长因子诱导2的受体自身磷酸化，并抑制表皮生长因子诱导的磷脂酶d活性和磷脂酶c - g的1酪氨酸磷酸化。
Phopholipase c - 1 ( plc - 1 ) is widely known to play an important role in regulating cell proliferation and differentiation , development of the organisms , cell transformation and oxidative stress . till now , the mechanism how phopholipase c - 1 acts can not be thoroughly illustrated , nor has the interaction between plc - 1 pathway and other signal pathways been systematically reported . this research chose 2 - de + ms as the basic method from all kinds of proteomics strategies and compared the total protein expression map of mef genetically deficient in plc - 1 ( plc - 1 - / - ) to that of wild type mef ( plc - l + / + ) aimed to find some protein spots differentially expressed , thus we can discuss the impact of knockout of plc - 1 on signal transduction initiated by growth factors such as egf comprehensively . in this way , we can study the biological function of plc - 1 and mechanism of plc - 1 pathway indirectly , which will contribute a lot to further analysis 鉴于plc - 1发挥上述作用的机制尚未完全阐明， plc - 1通路与其他信号通路间的交联和代偿尚无系统报道，又因为以往的研究方法不够全面，本研究以野生型小鼠胚胎成纤维细胞( plc - 1 ~ ( + / + ) )和缺失磷脂酶c - 1的小鼠胚胎成纤维细胞( plc - 1 ~ ( - / - ) )为研究模型，在众多蛋白组学策略中选择了双向电泳+质谱( 2 - de + ms )作为研究手段，通过对比表皮生长因子( egf )刺激24小时后上述两种细胞的总蛋白质表达差异，全面地探讨敲除plc - 1对生长因子诱导的信号传递的影响，从而间接研究plc - 1生物学作用、信号传递机制及其代偿情况，为后续的深入研究打下基础。
Especially , apoptin is partially similar with ribonucleotide reductase , uridylate kinase and phosphotriesterase in the amino acid sequence . the similarity between them indicates that apoptin maybe takes part in the signal pathway of apoptosis with phosphotriesterase activity or operates on hnrna with ribonucleotide reductase or uridylate kinase activity 凋亡素与它们的长序列相似性表明，它可能具有磷脂酶活性，在细胞信号通路中起作用；或者具有核糖核苦酸还原酶和尿苦酸激酶活性而对hnrna发挥作用；或者是与其中具有某种活性的蛋白质存在竞争性抑制作用。
