This result suggests that actin may participate the transportation of the acmnpv bv from nuclear to cytoplasm and getting out of the cell 根据实验结果，我们推测肌动蛋白可能参与了acmnpv出芽型病毒粒子mv ）由细胞质向细胞核运输以及从细胞膜排出的过程。
The study shows that f - actin skeleton system is responsible for the processes of infection , transportation , replication and assembling of the progeny virus 研究发现，许多病毒的侵染、运输、复制以及子代病毒粒子的装配等过程均与肌动蛋白骨架体系有关。
To investigate whether ha 132 is a structural component of hasnpv , western blot analysis of proteins in budded viruses ( bvs ) and occlusion derived virions ( odvs ) was conducted 对来自bv和odv的总蛋白质进行westernblot分析，结果表明ha132蛋白不是hasnpv病毒粒子的结构成份。
In order to know whether vp37 has the ability to combine nucleic acid , which was thought as the basic characteristic of plant virus mp , gel - retardation and uv - crosslinking assays were employed 病毒的移动蛋白具有结合核酸的能力，包括移动机制与管状结构和病毒粒子相关的移动蛋白。
Green fluorescences are major distribution of in cell plasms after 12h infection , and transferred into neucleus at 24h pi . this means that p78 / 83 is located at neucleus 结果表明，从感染后24h即可观察到p78 83 - egfp融合蛋白从细胞质定位转移到细胞核，并且随着感染的加深，融合蛋白在细胞核中被包装入病毒粒子。
It is found that the first , second and third types of connective tissue cells , which are analogous to crustacean haemocytes , are probably the originations of the renovation of epithelial cells 病毒粒子呈圆形，直径约100urn ，外有膜包被。在输卵管管壁上皮细胞中存在病毒粒于，是甲壳动物病毒具有垂直传播途径的一个重要证据。
Infection assay in vitro showed that overexpression of p78 / 83 had n ' t any evident effect on the virus growth and viral assemble . it is notable that the fusion protein can be assembled to virions . via actin dissemination in vac - orf9 - gfp infected cells , p78 / 83 - egfp might colocalize with actin 对vac - orf9 - gfp感染sf21细胞作电镜时相切片分析，结果表明，表达p78 83 - egfp融合蛋白的重组病毒，对病毒粒子的形态发生没有明显可见的影响。
Aliquots of cells were mixed 0 . 15 % mg / ml fb - 28 , and kept at 4c for 30min , fusion assays were conducted : fluorescence was measured immediately at regular time - points with fluorescence spectrophotometer with an excitation wave length of 560nm and emission wave length of 590nm . the percentages of membrane fusion was calculated . by monitoring fusion using the r18 assay , we found that the fluorescent brightener 28 influenced membrane fusion of virus and midgut epithelia cells 此外，采用分子探针r18 (荧光标记物)标记病毒囊膜，体外分离中肠上皮细胞，将标记的病毒粒子与离体中肠上皮细胞混合后保温，病毒吸附zh后，通过检测荧光的变化来监测病毒粒子与上皮细胞的融合。
First , sf9 and hzami cells were infected with successfully transfected supernatant . second a transfection - plaque method was used . with both methods , the production of infectious progeny virions was not observed . the results indicated that gp64 would not substitute for the function of ha133 通过其对hzami细胞的转染和上清对sf21细胞的感染及转染-空斑的方法，未检测到在hzami - hasnpv系统中产生有感染性的病毒粒子，表明gp64不能功能性地替代f蛋白ha133 ，并对这一结果进行了讨论。
The loop sequence of mb1 and mb2 were the anti sense and sense sequence ofing1 , respectively the sequence of mb3 was a piece of ssrna sequence in tobacco mosaic virus , which had no analogical to human gene . mbl was the most suitable probe because mbl had the highest fluorescence enhancemen after hybridizing wtth rna extrated froin normal cell 第三章，根据一种常见的病毒烟草花叶病毒( tmv )的核酸序列设计了分子信标荧光探针，由于tmv的遗传物质是rna ，分子信标又具有很高的特异性和灵敏度，因此感染了病毒粒子的植物叶片在经过简单处理后，可用分子信标检测叶片上。
