If we study on the phylogenetic relationship in intra - genus or intra - species using gapdh as a target gene , it may be make a discrepancy 对于距现今较近的动物，用其作为近亲缘关系的属内种间的标记基因，可能产生偏差。
Consequently the desirable gene may be linked to a marker gene , e . g . a gene conferring resistance to an antibiotic in the growth medium 如决定一种是否对培养基上抗菌素产生抗性的性状基因，就可作为标记基因用来判断外源基因是否转入。
( 2 ) the interest gene in pcar is under the control of acti which is the strong promoter in rice . the selectable marker gene is hyg gene ( 2 )表达载体pcar中目的基因由单子叶强启动子水稻act1启动子调控，选择标记基因为潮霉素抗性基因。
( 1 ) the intermediate vector pjar harbors acti promoter , regulating rip gene , and bar gene as selectable marker . this vector was used in gene gun transformation ( 1 )中间载体pjar含act1启动子调控的rip基因，选择标记基因为bar基因，可用于基因枪转化。
Sv40 polya signal and two loxp sites was added consecutively after the termination codon taa , and then a gfp gene was inserted between the two loxp sites 在三个终止密码子taa之后，连上sv40polya 。 sv40polya之后，又连上两个loxp位点。在两个loxp位点，插入标记基因gfp 。
5 . in this study , we have cloned camv35s and fmv promoter , nos terminater , mark gene nptii , and aim gene pat , epsps and cryia ( b ) genes used as positive collate 5 、克隆了camv35s 、 fmv启动子、 nos终止子、标记基因nptll 、抗除草剂基因epsps 、 pat 、抗虫基因cry1ao ）的阳性质粒作为转基因植物检测的阳性对照。
Bar gene also is one of widely used selective marker gene . the cloning of bar gene is important to plant transgenic engineering , gene expression and studying physiology 而且bar基因也是迄今为止应用最为广泛的一个抗除草剂选择标记基因，克隆出bar基因对植物的遗传转化，基因表达和生理学研究都有重要的作用。
As part of the project to improve isoflavone contents of legume forage by introducing isoflavone synthase ( ips ) gene , in this work , we have established the agrobacterium - mediated transformation system of white clover A311带有pbi121质粒，该质粒携有gus基因，其启动子为35s启动子，筛选标记基因为npt 。
Two are for use when marker allele frequencies in a study population are known , and two ( lcd } are for use when marker allele frequencies in a study population are not known and are only known in the extreme samples : ， ，乙娜用于群体的标记基因频率已知的情形，两个(赚i ， > 。和lcd ’ )用于群体的标记基因频率未知的情形。
Currently used plant transformation systems require selectable marker genes encoding antibiotic or herbicide resistance , along with the gene of interest , to select transformed cells from a large population of mostly untransformed cells 摘要在目前的植物转化系统中，要求在关注基因或目的基因转入细胞时，同时有标记基因存在。
