A white needle crystal was isolated and purified on systematic extraction of the alkaloid by silica gel column and gradient sublimation from oxytropis glabra 摘要采用生物?系统提取、矽胶柱层析分离及梯度升华技术从小花棘豆干草粉中分离出一种白色针状结晶。
The active agent was confirmed as oligosaccharide , mostly composed of glucose by pc , vc and acidhydrolysis . the active agent is stable between ph2 - 12 and below 90c 通过纸层析（ pc ）和柱层析（ vc ）分析，活性组分酸水解分析初步证实了其组分为葡萄糖为主要成分组成的寡糖类物质。
Nonstructural proteins can be pathogen - related proteins . we constructed the vectors that respectively express the fusion protein of x1 , x2 and green ( gfp ) or red fluorescent protein ( rfp ) in mammalian cells by recombinant pcr 目的蛋白以可溶形式存在胞浆中，经二步柱层析纯化后，可获得高纯度的rhigf - i 。
In the process of experiment , we separated better efficient antioxidative fractions by silica gel column chromatography with different extensible reagent , ethyl acetate : hexane ( 3 : 7 ) and chloroform : methanol ( 9 : 1 ) 实验中采用乙酸乙酯环己烷( 3 : 7 ) 、氯仿甲醇( 9 : 1 )为展开剂依次二次干柱层析分离出抗氧化物质。
By gel filtration on sepharose cl - 6b and cellulose acetate pellicle electrophoresis test , hpa and hpb were respectively a single strait symmetry apex and the result of electrophoresis assume a single spectrum strip 经sepharosecl - 6b柱层析和醋酸纤维素薄膜电泳检验， hpa和hpb均为单一狭窄对称峰且电泳呈单一谱带。
The best method for isolation and purification of - dihydroagarofuran sesquiterpene in celastrus angulatus is alternate normal - phase and reserve - phase high performance liquid chromatography 结果表明，对苦皮藤中富含二氢沉香呋喃倍半萜类化合物部分进行分离的过程中，采用正相、反相高压制备柱层析交替进行的方法最为有效。
Mm ) . mg2 + , mn2 + , zn2 + , fe2 + , fe3 + , cu2 + have enabled effect on enzyme activation and edta produce a strong inhibitory effect on the enzyme . embranch amino acid have no effect on the enzyme 在离子交换柱层析中，采用不同的ph值及不同类型的缓冲液对纯化条件进行优化，最终选择了ph6 . 0mes缓冲液，并得到了酶蛋白洗脱点为0 . 24 0 . 32mol lnacl 。
70kb and 130kb in size respectively . though pks gene cluster for antibiotics fr - 008 was cloned , the genes for amino - mycosamine residue remained unknown . thus , attempt was also made to clone the desired gene ( s ) , streptomyces sp 首先从大量的发酵液中提取分离了链霉菌fr - 008产生的抗生素fr - 008 ，并通过大孔树脂吸附、柱层析等手段进行了初步提纯。
Methylbenzyl amine as chiral auxiliaries to react with racimic ketorolac . then authors got optical amides . the diastereoisomeric mixture were separated by column - chroatography on silica gel . the results of separation depend on the structure of r 苯乙胺作为手性辅基，与外消旋酮咯酸生成一对光学活性的酰胺衍生物，再用简单易行的柱层析方法分离其非对映异构体。
4m phosphate buffer ; the second is that the crude enzyme runs on sephadex g100 - g50 column under the same experimental conditions ( only with sephadex g100 , g50 ) . at last polyacrylamide gel electrophoresis and sod assay tell us both of products are highly purified sod 因而不管从第一次的纯化来看，还是从文献上报道的来看，本实验的串联柱层析是较为合理有效的一种生产方法，值得推广。
