The main results and conclusions are show as follow : 1 . on the basis of the method of purification for ucp - 1 in rodent by lin et al . the ucp1 from adipose tissue in tree shrew was isolated and purified successfully 2 、中缅树? ucp1免疫学分析3 、中缅树? ucp1高效液相色谱分析主要研究结果和结论如下： 1 、在lin等人纯化ucp1的方法基础，利用sds银染法成功的分离出了ucp1 。
The plasmids pci - mbl54 containing full length of mutations mbl cdna were propagated hi escherichia coli xl - 1 blue , then the extracted and purified pci - mbl54 were used to transfect dhfr ( - ) chinese - hamster ovary ( cho ) cells . after screeened with g418 and cloned , 4 g418 - resistent clones were randomly selected for detection of mrna expression by rt - pcr and molecular beacons . it was found that all of the 4 positive cell clones expresse mbl analogue as detected in transcription level 抽提、鉴定、纯化重组质粒后，脂质体转染法将重组质粒导入中国仓鼠卵巢细胞( cho - dhfr ~ - )中， g418选择转染子并克隆化培养，经rt - pcr和分子灯塔探针杂交鉴定其mrna转录，获得4株稳定表达54位密码突变型mbl的cho细胞。
T cells were purified by nylon wool , the supertanant was collected after t cells were cultured for 48h , and stored at - 20 c . mrna in t cells which needed for rt - pcr reaction was isolate by trizol . results ( 1 ) the cd137 possitve rate on t cells from subacute senile mouse and aging mouse were lower than the normal 尼龙棉柱法纯化t细胞，培养，同时收集各组细胞培养上清，分别用正常t细胞培养上清加入衰老t细胞、衰老t细胞培养上清加入正常t细胞进行培养，双染法检测各组细胞中cd137表达率与细胞死亡的关系。
