The best tissue was the fin . the concentration of colchicines had great effect on the configuration of the chromosomes , and the concentration of 0 . 02 % is better . the concentration of the kc1 affected the crash of the cells , and had indirect effect on the dispersion of the metaphases 使用的秋水仙素的浓度对染色体的形态有较大的影响，最适的浓度为0 . 02 mol l ，使用的kcl低渗液的浓度决定着细胞破碎的程度，将对滴片的效果产生间接影响，相对来说，浓度为0 . 0375mol l要比浓度为0 . 075mol l的要好。
The transformed hairy roots were detected for agropine to make sure the transformation was successful . a conclusion was reached as follows : agrobacterium rhizogenes r1601 was an ideal strain for hairy roots transformation from trichosanthes bracteata voigt which exhibited extraordinary transformation rate ( 60 % ) . od600 = 0 . 7 was the optimum infection concentration for the tested transforming agent , with temperature at 25 , ph at 6 . 0 , 20min of infection and 48h of co - culture and phytohormone at 1 . 0mg / l being the most favourable hairy root - inducting pattern 通过实验和对结果的数据分析，得出下面的结论：发根农杆菌r1601是较好的大苞栝楼发根的诱导菌株，它对预培养24h的大苞栝楼子叶外植体具有最好的转化效率和发根诱导率( 60 ) ，最适的菌株感染浓度od _ ( 600 )为0 . 7 ，温度27 、 ph值为6 . 0 、 20min的感染时间和48h的共培养，以及黑暗条件和1 . 0mg / l的激素最有利于发根诱导的环境条件。
Once the recombinant virus has been i - dentified , we amplified the p - 1 stock to attain the large scale , high - titer viral stock in order to initiate expression studies . the recombinant angiostatin was produced from spodoptera frugiperda 9 ( sf9 ) insect cells infected by the high titer virus stock we have prepared . the time course for expression of recombinant protein was detected by sds - page and western blot , which can determine the optimal multiplicity of infection ( moi ) and the appropriate time of harvest for the protein 表达重组蛋白angiostatin ：用制备好的带有矗s标记的融合性angiostatin基因的高滴度重组杆状病毒贮存液感染草地贪夜蛾sfg细胞，用sds page电泳和hsternblot对感染不同时间后分泌的重组蛋白做时间表达分析，依此确定最适的感染复数（ moi ）和感染时间，以达到重组蛋白表达水平最适化，而后大规模进行重组蛋白的表达， sds page用来分析重组蛋白， westernblot用来在蛋白表达水平低的情况下检测表达的特异重组蛋白。
