Using ? 3 labeled with horseradish peroxidase , and o - ap as the substrate , an amperometric detection at - 150 mv ( vs sce ) results in a linear detection range of 1 . 17 - 35 . 5 ug - ml " 1 ( 1 )在第一章中报导了采用该技术，在低温下将补体第3成份抗血清固定在生物组分中。
The protein - storing cells in swietenia macrophylla were found to be populus - type , i . e . ordinary parenchyma cells containing both vacuolar protein inclusions and starch grains 用21kda蛋白质的抗血清进行兔疫印迹分析表明， 18kda蛋白质和21kda蛋白质有相同的抗原决定簇。
Li hong et al . expression of hyluronan receptors cd44 and rhamm in stomach cancers and their relevance with tumor progression . int j oncol , 17 : 927 - 932 , 2000 李宏等：小鼠野生型和突变型透明质酸受体rhamm的抗血清制备和初步应用。中华微生物学和免疫学杂志， 19 ： 54 ， 1999 。
( 2 ) in chapter 2 and 3 , renewable transferrin and complement 3 amperometric immunosensors have been developed for the determination of transferrin and complement 3 in human serum （ 3 ）在第四章中，采用溶胶描掖技术，在低温下将补体第三成分抗血清固定在生物组分中。
Compared with the known antibodies , the antiserum we prepared is highly specific and rules out the possibility of cross - reacting with angiotensin fragments . 4 与己有抗体相比，本实验制备的抗agt多克隆抗血清不仅具有较高的特异性，而且排除了与血管紧张素短片段的交叉反应。
This work produced large amounts of high - purity envelope protein antigens and envelope - specific antisera , and established detection systems for envelope antigens as well as antibodies 通过以上工作，得到了大量、高纯的被膜蛋白抗原和被膜蛋白特异的抗血清，建立了被膜蛋白抗原、抗体的检测体系。
The result manifest that there was no significant between over twice reinforcement vaccination and once reinforcement vaccination , on the base of background titers were no significant between the two field 因此制备抗血清的最佳免疫程序是， cdv 、 cav在屠宰前两周进行一次强化免疫， cpv进行两次强化免疫。
The assays lay a firm - foundation on detecting cmv fast and effectively and accumulate the experiences of efficient expressing exogenous gene in e . coli and preparation of antiserum 本实验结果为今后开展cmv的快速、有效的检测奠定了基础，同时为外源基因的大量表达及利用表达产物制备抗血清积累了一定的经验。
Utilizing the intensity adsorbing effect between the protein a and gold - electrode , and that , the protein a can combined the antibody ' s fc , and it is not influence the active of antibody 片断结合而不影响抗体的活性，在石英晶振表面固定转铁蛋白抗血清，研制了一种对于转铁蛋白有特殊响应的压电免疫传感器，在s
At the same time , we can conclude from our result : ucp1 and the rabbit anti - ucpl of tree shrew have a single tide - site . the content of rabbit anti - ucpl was about 1000 - 1000 , which was the better content 同时实验室结果还证明了ucp1与兔抗中缅树?抗血清为单位位点结合，而抗血清浓度在1000 10000倍检测效果最佳。
