Our final aim is to use the sensor to detect reactions of antibody - antigen in veterinary clinic and field . so it is very important to exam their applicability in veterinary 将电化学免疫传感器应用于兽医临床和现场的抗原抗体检测分析和诊断是我们的最终目的，因此测试其在兽医方面的应用具有特别重要的意义。
The products cover the fields of the study of molecule biology , expression of protein , from cell culture to layer purification , from antigen antibody , the whole set reagent kit to the microorganism culture and the research of theactivity of the biology 其产品覆盖了生命领域从分子生物学研究、蛋白表达、细胞培养到层析纯化，从抗原抗体、成套试剂盒到微生物培养及其生物活性研究等相关试剂几近所有过程。
The contents of the research mainly include three aspects : firstly , it is to select proper materials for designing and fabricating the electrochemical immunosensor . secondly , using the proper method to set up the experiment conditions and judge the characters of the sensor . thirdly for examining the result of the electrochemical immunosensor made , we use the sensor to detect antibody - antigen reactions of several common diseases of parasites and microbes 本研究的内容主要包括3个方面：一是筛选适当的材料，设计和构建电化学免疫传感器；二是采用适当的方法建立电化学传感器的实验条件，并测定传感器的各种性能；三是应用电化学免疫传感器检测几种常见寄生虫病、传染病的抗原抗体反应，了解研制的电化学免疫传感器的实用效果。
We have designed and fabricated a kind of electrochemical immunosensor by using the theory of immunology and the technology of electrochemistry and apply the sensor to detect reactions of antibody and antigen on veterinary . by this way , we surpose to set up a new quick , sensitive , specific detecting method of antibody - antigen reactions for the veterinary practice use 我们利用经典的免疫学理论和成熟的电化学技术，设计和构建了一种电化学免疫传感器，将其应用于兽医方面的抗原抗体反应检测分析，试图在兽医领域建立一种新的快速、灵敏、准确的抗原抗体反应检测、诊断方法，供生产上使用。
A piezoelectric immunosensor was developed by immobilized transferring antiserum on the surface of quartz crystal to especial answer transferring , it can be get good answer to determined in the range of 3 . 92 ~ 79mg / l ; the adsorption and desorption of consult serum on the quartz crystal monitored with tr - antibody , determined the rate constant of immuno - reaction was 2 . 14xl07 ( mol / l ) ' 1xs ' 1 , the association constant of the immuno - reaction ( k1ss ) was 6 . 75xl07 ( mol / l ) ~ l Oz vomg l范围内的参考血请中的转铁蛋白进行了定量测定，获得很好的响应；利用石英晶体微天平技术在线监测了参考血清在固定了转铁蛋白抗体的石英晶振上的吸附与解吸过程，测得了其抗原抗体免疫反应的速率常数为2 14xl0 ’ （ mol l ） ’ xs “ ‘ ，结合常数为6
Methods according to theory of specific binding of antigen and antibody , at first the anti - a monoclonal antibody ( ma ) and anti - bma were labeled with the fluorescent , then fluorescent - labeled antibodies ( fla ) were bound with corresponding biological material ( such as bloodstain ) in the optimum condition , finally the abo blood type of bloodstain was determined under microscope fluorescent 方法根据抗原抗体特异性结合的原理，首先对抗a 、抗b单克隆抗体进行荧光标记，然后使荧光标记抗体与相应抗原（血痕）在最佳条件下结合，最后荧光显微镜镜检，判定血痕的血型。
In this study we had used the sensor to detect antibody - antigen reactions of fasciolsis , nd , ibd and eds . it is proved the sensor is fitted to use in detecting antibody - antigen reactions of these diseases . the results of detecting antibody - antigen reactions of fasciola gegatica , nd , ibd and eds by using the sensor basically conform to that with standard samples 在实验研究中，我们先后应用电化学免疫传感器测试了大片形吸虫，鸡新城疫( nd ) 、鸡传染性法氏囊病( ibd ) 、鸡减蛋综合征( eds )等的抗原抗体反应，结果与我们先前初步设定的判断标准基本相符。
Ptxb1 - hng and ptxb1 - m - insulin are expressed in e . coli successfully . after sds - page and densitometric scan analysis , the results show that the expression level of hng fusion protein is above 40 % and m - insulin fusion protein above 50 % of total bacterial proteins . western - blot result demonstrated m - insulin fusion protein had specific reaction with mouse anti human insulin antibody ( igg ) Pbv220 ? hng在大肠杆菌中未检测到表达，后两个克隆在大肠杆菌bl21 ( de3 )中获得高效表达， hng及m - insulin融合蛋白表达量分别占全菌蛋白的40及50左右；经western - blot鉴定m - insulin融合蛋白可以与小鼠抗人胰岛素单克隆抗体( igg )发生抗原抗体结合反应。
Piezoelectric sensing technique has been applied to many fields , including analytical chemistry , life science , environmental monitoring and surface sciences because of it ' s small size , high sensitivity , simplicity , low power consumption and broad sensing spectrum . piezoelectric immunosensors possess high selectivity provided by antibodies or antigens and sensitivity response directly to the mass change of proteins . immobilization technologies of biomolecules are of paramount importance in order to preserve their biological activity 压电免疫传感技术利用压电晶体与抗原抗体特异性免疫反应结合的特征，通常在压电石英晶体表面先固定抗原(抗体) ，响应溶液中抗体(抗原)引起的质量、密度和粘度等性质的变化，它兼具有压电质量响应的高灵敏性和生物化学反应的高特异性。
Through the primary studies , our sensor has showed many excellent characters such as adaptability and specificity , quick detection , simple operation , small amount consumption of sample serums , miniature of the sensor , convenience to carry and the detecting instrument is cheap and so on . with the many excellent and special characters the sensor has displayed its light apply perspectives . we believe through further designing and experiments it is possible to use the sensor to quick detect reactions of antibody - antigen and diagnose diseases in veterinary clinic and fields 本研究构建的电化学免疫传感器经初步的实验研究就显示了其许多优良特性：传感器适用于不同类型的抗原抗体反应检测，具有良好的稳定性和特异的检测效果；检测快速，操作简便；样品血清用量少；传感器微型巧小，携带方便，检测仪器价格便宜等优点，预示了其良好的应用前景，我们相信，通过进一步的设计和实验研究，就有可能将传感器应用于临床和现场抗原抗体反应快速、准确检测分析和疫病诊断。
