Immunoassay and antifreeze detection activity of microdera punctipennis dzhungarica fusion antifreeze protein expressed in escherichia coli 准噶尔小胸鳖甲融合抗冻蛋白的免疫检测和抗冻活性分析
L to get the expression vector for the antifreeze protein gene followed by importing it into the caluus of maize by gene - gun 将此表达盒连接到pbi121 . 1上，最终获得了抗冻蛋白基因植物高效表达载体pbrtsafp 。
The antifreeze proteins have been found in many organisms , such as fishes , plants and insects and the antifreeze proteins in fishes are studied in detail 其中研究比较清楚的是鱼类的抗冻蛋白，对植物和昆虫抗冻蛋白的研究也正在开展。
At last , we detected the expression of gus gene linked to antifreeze protein gene . the result indicates that gus gene is expressed strongly and jnstantaneous ] y in ca ] uus of maize 用基因枪法将之导入玉米的愈伤组织中，并检测了与抗冻蛋白基因连在一起的gus基因的表达情况。
Antifreeze proteins ( afps ) are special proteins that have the ability to inhibit ice crystal growth , which depresses the freezing point of body fluid , thereby allowing survival at subzero temperatures . afps are firstly found in polar fishes , and then isolated from insects and plants 抗冻蛋白( antifreezeproteins ， afps )是一类能使生物体耐受低温的特殊蛋白质，首先在极区鱼类中发现，之后又从昆虫和植物等中被分离出来。
The antifreeze proteins have many applications in increasing the quantity and quality of crop , improving the seedling liviability , prolonging the growth - season of crop and extending the planting - range , as well as decreasing the stored temperature of crop to prolong the stored time 抗冻蛋白有很多重要的应用，它可以改进农作物的产量和质量，增强幼苗的存活力，延长作物的生长季节和栽种范围，还可以降低作物的贮存温度，增加贮存的时间。
After careful studying their relative importance to immune response and the possibility of the match , seventeen sequences of interest were selected for further experiment , including estss analogous to 11 . 5kd antibacterial peptide , lysozyme , serine protease and its inhibitor , lectin , antifreeze protein , et al . primers designed according to the sequences were used to amplify the corresponding estss from both blood and cephalothorax cdna library 在仔细分析了它们在免疫系统中的重要性和在对虾中出现的可能性之后，从中选出了17条可能编码抗菌肽，溶菌酶，凝集素、丝氨酸蛋白酶及其抑制剂，抗冻蛋白等蛋白质的序列，以此为依据设计引物，在中国对虾的血液和头胸部cdna文库中扩增相应的序列。
Now it has been one of the most important aquatic products in the freshwater cultivation . however , this prawn ca n ' t survive at a water temperature lower than 14c , which has seriously limited its cultivation expanding . in order to obta in a new breed of this prawn with increased cold - resistance , we investigated the cloning of a synthetic gene ( sbwafp ) based on the primary sequence of the mature spruce budworm ( choristoneura fumiferana ) antifreeze protein ( sbwafp ) and the integration of sbwafp into the embryo genomes of giant freshwater prawn by spermatophore - microinjection ( smi ) , a sperm - mediated gene transfer technique 本研究的特色和创新之处在于，针对罗氏沼虾不耐低温，但体型相对较大，精荚明显的特点，首次将目前已知具有最强抗冻活性的云杉卷叶蛾( sprucebudworm ， choristoneurafumiferana )抗冻蛋白( sbwafp )基因( sbwafp ) ，通过精子介导的转基因技术整合到罗氏沼虾的胚胎中，以期培育出耐低温的罗氏沼虾新品系。
Ln our experiment , a lot of regulator sequences inc1uding the 35s promoter with double enhancer e1ements and the terminator nos , the fl sequence and kozak sequence for improving the genetic statement in the level of translation , were appended on the two s ides of the artifically pseudopleuronectes americanus antifreeze protei n gene by a series of process , and the products were linked into pbil21 本实验中人工合成了美洲拟鲽抗冻蛋白基因，并在美洲拟鲽抗冻蛋白基因两侧添加了许多调控序列，如具有加倍增强子的35s启动子和nos终止子，它们可以促进抗冻蛋白基因的转录，及可在翻译水平上提高基因表达的序列和kozak序列，从而构建了抗冻蛋白基因的高效表达盒。
