Purification and renaturation of recombinant human cu , zn - sod by metal - chelating affinity chromatography 金属螯合亲和层析纯化和复性重组人铜锌超氧化物歧化酶
To study the renatural condition of r hpf4 , we adopted air oxygenation method to make r hpf4 refolding correctly 研究重组hpf4的复性条件，采用空气氧化法使重组hpf4正确重折叠。
The choice of an expression system for the high - level production of recombinant proteins depends on many factors 建立hpk - 5因子分离纯化工艺； 4建立体外检测复性蛋白质活性的方法。
Time - scanning fls indicated the process of denaturing by urea was faster than that of recovering its activity 荧光时间扫描光谱表明cml的腺变性是一个相对较快的过程，而腺变性的复性则是一个相对缓慢的过程。
When the denatured reduced egg white lysozymes were renatured by dilution method , an observable amount of aggregate precipitate could be immediately formed 当用复性液稀释复性还原服变性蛋白溶菌酶时，会迅速产生可观量的沉淀。
We have used two different operating schemes , fed - batch and step - addition , independently and collaboratively to refold denatured lysozymes 本研究中，我们利用馈料式与间歇式不同的复性方法，以独立地或结合地方式进行溶菌酶之复性。
Inhibition experiment of blood vessel proliferation in the chicken chorioallantoic membrane was used to study the biocharacter of r hpf4 and renaturation effect 采用鸡胚绒毛尿囊膜血管生成抑制实验观察重组hpf4的血管生成抑制活性及复性效果。
Various strategies , including the direct dilution method , have been utilized to enhance the performance of the refolding process ; however , refinement and improvement on these methods are ongoing 直接稀释法是复性程序中最简单的方法，但如何提高其复性效率仍在持续研究中。
After sonification , the precipitate was washed repeatedly by ionic and non - ionic detergents to remove membrane proteins as could as possible , and the cleaner inclusion bodies were abtained 其次对包涵体体外复性进行研究。用离子型与非离子型去污剂反复洗涤包涵体，尽可能去除膜蛋白。
After dialysis , the recombinant gst - ri was purified by rnase a - sepharose 4b affinity chromatography , and the single band of gst - ri was showed on a sds - electrophoresis gel to remove the extra urea 复性的gst一班经knasea - sepharose4b亲合层析纯化， sds一page鉴定得到单一的gst一rj条带。
