Aim to construct human antisense cd40 rna eukaryotic expression vector and investigate its effects on functions of b lymphocytes . methods partial sequence of human cd40 gene was obtained by rt - pcr . then , the pcr product was cloned with a t - a clone technique to construct cd40 / pucm - t 方法从健康人外周血单个核细胞提取总rna ，利用rt - pcr扩增cd40部分序列，用t - a克隆技术形成pcr产物的克隆cd40 pucm - t ，并进行基因测序。
By means of discontinuous tris - hcl buffer system and poly aery 1 amide gel electrophoresis ( page ) , zymogram of 23 species of 11 genera in 4 subfamilies mirinae , orthotylinae , phylinae and deraeocorinae are gained . by specific primer pcr and sequencing , 57 cyt b gene 433bp sequences are obtained from 34 species respectively belonging to 17 genera in 5 subfamilies mirinae , orthotylinae , phylinae , deraeocorinae , bryocorinae from 64 species of 23 genera which involved in this research , and 2 outgroup species of family anthocoridae as well . 1 首次通过特异引物扩增和基因测序的研究方法从被试的5亚科23个属的64种盲蝽中获得了盲蝽亚科、合垫盲蝽亚科、叶盲蝽亚科、赤爪盲蝽亚科和蕨盲蝽亚科bryocorinae5个亚科17个属的34种盲蝽以及外群花蝽科anthocoridae2种花蝽的cytb基因序列57条。
The dna sequence of the amplified fragment is identical with the published sequence of gene ubia in genbank with blast , apart from that the 1 , 007th base was mutated from t to c . conclusions : a rapid mensuration of the content of coqio in microbial cells has been set up in this study 87 。 （ 4 ）成功克隆了ubia基因，将该基因测序结果与genbank中序列进行blast ，结果显示，除第1007个碱基与genbank上发布的ecoltmc400的ubia序列相应碱基不同（ t ? c汐其余碱基序列完全相同。
( d ) it is strongly suggested that subfamily mirinae should be revised because its remarkable heterogeneity . 3 . for the first time , cyt b gene 432bp sequences of species belonging to genera of 5 subfamilies and 2 outgroup species are obtained in this research which is more than 50 % of 64 species involved in the experiments 3 、首次在盲峙科昆虫中通过特异引物扩增和基因测序的研究方法获得了盲峙科5个亚科门个属34种盲峙和2个外群种类的cytb基因433hp序列，占被试23个属64种盲峙的50以上。
