Normal tissues and tumor tissues methods a gene was cloned by exon trapping and exon linking technique . homologous analysis of the gene was researched by blast . then we explored the gene expression pattern in various human tissues by northern blot and rt - pcr 正常组织及肿瘤组织实验方法利用捕获的外显于进行blast同源性分析，之后采用外显于拼接技术克隆新基因，利用rtpcr及northern印迹杂交检测该基因的组织表达情况，同时以p 《 ctin作内对照。
L lp - hsdl ma of i . 5kb was detected in the rna extracted from cult ' ured chorionic trophoblast using northem blot hybridhation method . the expression of ll6 - hsdl wa was induced by treating the chorionic cells with dexamethasone ( l0 - ' , l0 ' m ) for 24h , and the induction was blocked by co - treatinent of the cells with ru486 . these results suggest a gr - mediated effect of glucocorticoid on the rnrna expression and reductase activity of l l 9 - hsd1 人h合成的肌?一加x门’ m ）对培养的绒毛膜滋养层细胞的11p hsdi还原酶活性具有诱导作用，此诱导作用可以被gr阻断剂ru486门0川）所阻断； northern印迹杂交方法进一步证实， dex亦可诱导绒毛膜滋养层细胞11p叫inrna的表达，此诱导作用也可以被ru486门0叫川）所阻断
