In the first trial , combination of enzymatic digestion was used to prepare suspensions of spermatogenic cells from adult mouse testis , and then a modified discontinuous percoll gradient centrifugation method ( 15 % , 22 % , 30 % , 40 % , 50 % , 60 % ) was introduced to isolate spermatids from the cellular suspensions . the content of spermatids in each isolated fraction by percoll method was determined by morphology ( wright - giemsa stain ) and flow cytometry analysis , and the viability of spermatogenic cells was assessed by using eosin y exclusion test 在第一部分试验中，首先利用连续3次组合酶消化成年小鼠睾丸制备睾丸细胞悬液，然后经6层非连续percoll梯度离心法( 15 、 22 、 30 、 40 、 50和60 )分离，通过形态学和流式细胞术鉴定南京医科大学硕士学位论文各个percoll组分中精子细胞的含量，并以伊红y排斥试验测定细胞的存活率。
Blasting the nucleotides sequences of hsei and hseii with data of genbank , we locate them in 11q24 . nine exons of them are same , but two exons of hsei and one exon of hseii are different . it hints that hsei and hseii are two transcripts of hse gene transcribed from the different transcription start sites 通过在genbank中南京医科大学颀士研究主毕业论义查询， hsei和hsell定位于人n号染色体长臂的2区4带，它们在基困组上的9个外元是一致的，但是hsei的前两个外元和hsell的第一个外元是不同的。
Yherefore , many researchers have shown great concern with the development of vaccines to against schistosomiasis . at early stage , the research of vaccines of schitosomiasis was centered on dead vaccine and athenuated cercaria of schistosomes while molecular vaccine and athenuated cercaria of schistosomes while molecular vaccine is currently the focus of research with anti - infection protective immunity as its main concern . guan xiaohong and zhao weixian ( 1986 ) certified that the allergen of egg granulonma of schistosoma japonicum might firstly come from gut associated antigen ( gaa ) of schistosomula and adult worm and that daa had cross reaction with soluble egg antigen ( sea ) and membrane associated antigen ( maa ) ; and the gaa of schistosoma japonicum might play a sensitizing role in egg granuloma formation Np30主动免疫c57bl 6对尾蚴攻击感染产生42 . 05的保护力，肝组织减卵率为66 . 63 ； balb c和昆明种小鼠的保护率分别为39 . 53和50 . 46 ；免疫山羊可诱导42 . 78的减虫率，肝组织减卵率为35 . 83 ，粪减卵率为25 ，并可明显抑制肝脏虫卵肉芽肿的大小，肉芽肿数量明显减少，纤维化减轻，体重明显增加，因此np30是南京医科大学博士学位论文很有希望的抗日本血吸虫病疫苗侯选分子。
