At present , the main methods used in the study on chinese traditional medicine in gastrointestinal dynamics animal experiment are gastric emptying and small intestine motional rate mensuration , parent electric bridge strain gauge mensuration , gut hormone determination ( gas , mot , ss et ) , immunohistochemical method , excised gastrointestinal smooth muscle dynamoscopy , intragastric and intestine pressure tracing 摘要中药在胃肠动力动物基础实验研究，目前主要采用胃排空小肠推进率测定法、胃肠道腔外电桥式应力传感器记录法、胃肠激素测定（胃泌素、胃动素、生长抑素等） 、免疫组织化学方法、离体体胃肠平滑肌肌条动力测定、胃肠内压描记法。
Using colophony - paraffin ( cp ) embedding tissue section technique and immunohistochemical streptalidin - peroxidase ( sp ) method , we have investigated the distribution of 5 - ht neurons in the brain of camponotus japonicus ( big worker ant ) , compared the immunoreactivity with gaba , and primarily discussed the character of 5 - ht distribution . the results show that 5 - ht immunoreactive processes originate from a relatively small number of cell bodies but each neuron has processes over a large volume of the neuropil of the brain 本实验采用树脂石蜡( cp )组织包埋切片技术和链霉菌抗生物素蛋白-过氧化物酶( sp )免疫组织化学方法，研究了5 - ht能神经元在日本弓背蚁( camponotusjaponicus )大工蚁脑中的分布情况，并与gaba的分布进行了比较，初步探讨了5 - ht在各个脑区的分布规律和特点。
The immunohistochemical method and western blot were preformed to show the expression of egfr and rt - pcr method was applied to determine the expression of egfr mrna in placenta trophoblast of pregnancy with pih . 3 . egfr of jar was determined by immunohistochemical method and egfrmrna was analyzed by rt - pcr method 电子显微镜观察正常妊娠及妊高征患者两组胎盘滋养细胞的超微结构，应用免疫组织化学方法及westernblot方法测定妊高征胎盘滋养层细胞表皮生长因子受体（ egfr ）的表达，以及rt一nr技术对egfrmrna进行定量分析。
In this study , the expression of nfkb p65 and ikba ( inhibitory kappa b alpha , ikba ) in poly - morphonnucleared cells , mononuclear cells and fibroblastic cells during rat skin contusion repair will be investigated using immunohistochemical technique and the relationship with contusion will be revealed , which can provide the theorical evidence to identify the contusion age I b因其抑制nf b活性，也越来越受到人们的重视，故本实验应用免疫组织化学方法观察nf b家族的重要成员nf bp65与其抑制因子i b在大鼠皮肤挫伤后不同时间在多核粒细胞、单核巨噬细胞及成纤维细胞中的表达变化，揭示其与皮肤挫伤时间的关系，为皮肤挫伤时间的判定提供新的理论依据。
$ mx # ( 2bo2 ) ffr $ $ htet # $ nq9i & kk $ 4n8 $ $ 4ta scf mana $ ismffs whether s1eep deprivation cou1d induce neuron pro1iferation has not got definite conclusion so far . in the present research , we performed small pedestal s1eep deprivation method to remove rapid eye movement s1eep ( rems ) . we observed the neuron pro1iferation and differential in immunohistochemistry and doub1e 1abeling iariunohistochemistry staining , and the expression of stem ce11 factor mrna in situ hybridization technique after s1eep deprivation in rat hippocampus 本研究应用细胞增殖标记brdu cna免疫组织化学方法结合双重标记兔疫组织化学技术，观察睡眠剥夺对成熟大鼠脑内神经元增殖及分化的影响，应用原位杂交技术观察了睡眠剥夺大鼠海马神经细胞scf基因inrna表达的变化，对睡眠剥夺引起海马神经细胞增殖的分子机制作了初步探讨。
Using cp embedding tissue section technique and immunohistochemical method , we have investigated the distribution of gaba and gabaergic neurons in the females and males of camponotus japonicus and compared them and primarily discussed the function of gaba . protocerebrum shows pronounced gaba - like immunoreactivity . the three neuropile regions of the optic lobe are brown and take on obvious stratification 本实验采用cp包埋切片技术，结合免疫组织化学方法，对日本弓背蚁( camponotusjaponicus )雌蚁和雄蚁脑中gaba和gaba能神经元的分布进行了较详细的研究，比较了雌蚁和雄蚁的异同，并对gaba在各个脑区的作用机理进行初步探讨。
In order to investigate the ontogeny of the pituitary prolactin ( prl ) expression of sheep fetuses during gestation , the pituitary glands were collected at days 60 , 90 , 120 of gestation , the first day after birth , respectively . the cells containing the prolactin ( prl ) were detected using immunohis ' ochemistry method 为了研究绵羊胎儿垂体中催乳素细胞( lactotrophs )的发育和功能变化，分别在胎儿发育的第60 、 90 、 120天和出生期采集垂体，利用免疫组织化学方法研究了垂体中催乳素( prolactin ， prl )的表达及其变化。
The lung tissue for immunohistochemitry and laser scanning confocal microscopy were fixed and embedded . the morphological alteration of pulmonary neuroendocrine cells which stain for calcitonin gene - related peptide ( cgrp ) , serotonin ( s - ht ) and luteinizing hormone ( lh ) were studied . the results of these were dealed with computer image analysis and statistical treatment 肺组织取材后经固定、梯度酒精脱水、包埋、连续切片后，应用免疫组织化学方法、透射电镜及激光扫描共聚焦技术观察了降钙素基因相关肽( cgrp ) 、五羟色胺( 5 - ht ) 、黄体生成素( lh )阳性细胞的随龄变化，并对实验结果进行了计算机图像分析和统计学处理。
