The cultured cell suspensions tested by western - blotting showed that transfected cells could express the exogenous gene and secrete human lactoferrin protein , with mw of 34 kd . the highest amount detected with elisa reached 65mg / l medium / 105 cells . the recombinant hlf protein has the effect of inhibiting e . coli proliferation , whose activity is 1 . 4 - 1 . 8 times higher than the commercially available hlf 诱导后，培养液上清通过western - blotting分析证明，转染细胞表达并分泌出人乳铁蛋白，分子量为34kd ； elisa检测重组蛋白最高表达量为65mg l培养基10 ~ 5细胞；抗菌实验表明，所获得的重组人乳铁蛋白具有抑制大肠杆菌生长的作用，而且比人乳铁蛋白标准品作用更强。
Whey protein is biological active protein extracted from milk with high biological utilization value rich of immunoglobulin , lactoferrin , cgmp and other active eelements , and whey protein is famed as “ king of protein ” for the high purity and absorption rate and best amino acid composition 乳清蛋白是从牛奶中提取出来的生物活性蛋白，具有很高的生物利用效率，富含免疫球蛋白、乳铁蛋白、糖巨肽等多种活性成分，以其纯度高、吸收率高、氨基酸组成最合理等诸多优势被推为“蛋白之王” 。
